Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2246 and Shew_2247 overlap by 23 nucleotides Shew_2247 and Shew_2248 are separated by 0 nucleotides Shew_2248 and Shew_2249 are separated by 163 nucleotides Shew_2249 and Shew_2250 overlap by 4 nucleotides
Shew_2246: Shew_2246 - pseudouridine synthase (RefSeq), at 2,625,784 to 2,626,665
_2246
Shew_2247: Shew_2247 - putative transcriptional regulator (RefSeq), at 2,626,643 to 2,627,275
_2247
Shew_2248: Shew_2248 - chromosome segregation and condensation protein ScpA (RefSeq), at 2,627,276 to 2,628,064
_2248
Shew_2249: Shew_2249 - Sua5/YciO/YrdC/YwlC family protein (RefSeq), at 2,628,228 to 2,628,848
_2249
Shew_2250: Shew_2250 - phosphotransferase domain-containing protein (RefSeq), at 2,628,845 to 2,629,705
_2250
Position (kb)
2627
2628
2629 Strain fitness (log2 ratio)
-2
-1
0
1 at 2626.420 kb on + strand, within Shew_2246 at 2626.629 kb on - strand at 2627.504 kb on - strand, within Shew_2248 at 2627.678 kb on - strand, within Shew_2248 at 2627.688 kb on + strand, within Shew_2248 at 2627.696 kb on - strand, within Shew_2248 at 2628.562 kb on + strand, within Shew_2249 at 2628.576 kb on - strand, within Shew_2249 at 2628.756 kb on + strand, within Shew_2249
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,626,420 + Shew_2246 0.72 -0.9 2,626,629 - -2.0 2,627,504 - Shew_2248 0.29 -1.0 2,627,678 - Shew_2248 0.51 -1.0 2,627,688 + Shew_2248 0.52 -2.0 2,627,696 - Shew_2248 0.53 -0.8 2,628,562 + Shew_2249 0.54 -1.1 2,628,576 - Shew_2249 0.56 +0.2 2,628,756 + Shew_2249 0.85 +0.4
Or see this region's nucleotide sequence