Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_R0069 and Shew_R0070 are separated by 20 nucleotides Shew_R0070 and Shew_2038 are separated by 61 nucleotides Shew_2038 and Shew_2039 are separated by 293 nucleotides Shew_2039 and Shew_2040 are separated by 154 nucleotides
Shew_R0069: Shew_R0069 - tRNA-Val (RefSeq), at 2,350,595 to 2,350,671
_R0069
Shew_R0070: Shew_R0070 - tRNA-Val (RefSeq), at 2,350,692 to 2,350,768
_R0070
Shew_2038: Shew_2038 - hypothetical protein (RefSeq), at 2,350,830 to 2,351,273
_2038
Shew_2039: Shew_2039 - GCN5-related N-acetyltransferase (RefSeq), at 2,351,567 to 2,354,269
_2039
Shew_2040: Shew_2040 - diguanylate cyclase (RefSeq), at 2,354,424 to 2,355,467
_2040
Position (kb)
2351
2352
2353
2354
2355 Strain fitness (log2 ratio)
-1
0
1
2 at 2351.032 kb on - strand, within Shew_2038 at 2352.080 kb on + strand, within Shew_2039 at 2352.088 kb on - strand, within Shew_2039 at 2352.306 kb on + strand, within Shew_2039 at 2352.314 kb on - strand, within Shew_2039 at 2353.011 kb on + strand, within Shew_2039 at 2353.705 kb on + strand, within Shew_2039 at 2353.713 kb on - strand, within Shew_2039 at 2353.737 kb on + strand, within Shew_2039 at 2353.737 kb on + strand, within Shew_2039 at 2354.664 kb on + strand, within Shew_2040 at 2355.244 kb on + strand, within Shew_2040
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,351,032 - Shew_2038 0.45 -0.8 2,352,080 + Shew_2039 0.19 +0.4 2,352,088 - Shew_2039 0.19 +0.8 2,352,306 + Shew_2039 0.27 -0.2 2,352,314 - Shew_2039 0.28 +0.3 2,353,011 + Shew_2039 0.53 -0.5 2,353,705 + Shew_2039 0.79 +0.0 2,353,713 - Shew_2039 0.79 +2.1 2,353,737 + Shew_2039 0.80 +0.2 2,353,737 + Shew_2039 0.80 +0.1 2,354,664 + Shew_2040 0.23 +0.8 2,355,244 + Shew_2040 0.79 +0.2
Or see this region's nucleotide sequence