Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1988 and Shew_1989 are separated by 110 nucleotides Shew_1989 and Shew_1990 are separated by 6 nucleotides Shew_1990 and Shew_1991 overlap by 4 nucleotides Shew_1991 and Shew_1992 overlap by 4 nucleotides Shew_1992 and Shew_1993 are separated by 55 nucleotides
Shew_1988: Shew_1988 - cytochrome c oxidase, cbb3-type, subunit III (RefSeq), at 2,299,988 to 2,300,959
_1988
Shew_1989: Shew_1989 - hypothetical protein (RefSeq), at 2,301,070 to 2,301,549
_1989
Shew_1990: Shew_1990 - heavy metal translocating P-type ATPase (RefSeq), at 2,301,556 to 2,303,979
_1990
Shew_1991: Shew_1991 - cytochrome oxidase maturation protein, cbb3-type (RefSeq), at 2,303,976 to 2,304,161
_1991
Shew_1992: Shew_1992 - hypothetical protein (RefSeq), at 2,304,158 to 2,304,850
_1992
Shew_1993: Shew_1993 - fumarate/nitrate reduction transcriptional regulator (RefSeq), at 2,304,906 to 2,305,655
_1993
Position (kb)
2301
2302
2303
2304 Strain fitness (log2 ratio)
-2
-1
0
1 at 2301.326 kb on - strand, within Shew_1989 at 2302.498 kb on - strand, within Shew_1990 at 2303.529 kb on - strand, within Shew_1990 at 2304.173 kb on - strand at 2304.475 kb on + strand, within Shew_1992 at 2304.475 kb on + strand, within Shew_1992 at 2304.488 kb on - strand, within Shew_1992 at 2304.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,301,326 - Shew_1989 0.53 -1.2 2,302,498 - Shew_1990 0.39 -0.9 2,303,529 - Shew_1990 0.81 -1.6 2,304,173 - +0.2 2,304,475 + Shew_1992 0.46 -0.5 2,304,475 + Shew_1992 0.46 +0.2 2,304,488 - Shew_1992 0.48 -1.9 2,304,944 - -0.8
Or see this region's nucleotide sequence