Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1920 and Shew_1921 are separated by 291 nucleotides Shew_1921 and Shew_1922 are separated by 44 nucleotides Shew_1922 and Shew_1923 are separated by 158 nucleotides Shew_1923 and Shew_1924 are separated by 319 nucleotides
Shew_1920: Shew_1920 - alpha/beta hydrolase fold (RefSeq), at 2,217,009 to 2,217,776
_1920
Shew_1921: Shew_1921 - replication initiation regulator SeqA (RefSeq), at 2,218,068 to 2,218,598
_1921
Shew_1922: Shew_1922 - phosphoglucomutase (RefSeq), at 2,218,643 to 2,220,322
_1922
Shew_1923: Shew_1923 - peptide methionine sulfoxide reductase (RefSeq), at 2,220,481 to 2,220,960
_1923
Shew_1924: Shew_1924 - succinylglutamate desuccinylase (RefSeq), at 2,221,280 to 2,222,347
_1924
Position (kb)
2218
2219
2220
2221 Strain fitness (log2 ratio)
-2
-1
0
1 at 2217.973 kb on + strand at 2219.490 kb on - strand, within Shew_1922 at 2219.772 kb on + strand, within Shew_1922 at 2219.848 kb on - strand, within Shew_1922 at 2220.388 kb on - strand at 2220.824 kb on + strand, within Shew_1923 at 2220.842 kb on - strand, within Shew_1923 at 2221.086 kb on + strand at 2221.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 2,217,973 + -1.6 2,219,490 - Shew_1922 0.50 -0.8 2,219,772 + Shew_1922 0.67 -1.9 2,219,848 - Shew_1922 0.72 -0.4 2,220,388 - -1.1 2,220,824 + Shew_1923 0.71 -0.9 2,220,842 - Shew_1923 0.75 -1.0 2,221,086 + -0.1 2,221,235 + +0.4
Or see this region's nucleotide sequence