Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1150 and Shew_1151 are separated by 216 nucleotides Shew_1151 and Shew_1152 are separated by 29 nucleotides Shew_1152 and Shew_1153 are separated by 81 nucleotides Shew_1153 and Shew_1154 are separated by 23 nucleotides
Shew_1150: Shew_1150 - transmembrane pair domain-containing protein (RefSeq), at 1,324,509 to 1,324,931
_1150
Shew_1151: Shew_1151 - OmpA/MotB domain-containing protein (RefSeq), at 1,325,148 to 1,325,852
_1151
Shew_1152: Shew_1152 - MscS mechanosensitive ion channel (RefSeq), at 1,325,882 to 1,326,706
_1152
Shew_1153: Shew_1153 - no description, at 1,326,788 to 1,326,934
_1153
Shew_1154: Shew_1154 - hypothetical protein (RefSeq), at 1,326,958 to 1,327,611
_1154
Position (kb)
1325
1326
1327 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1324.925 kb on + strand at 1325.090 kb on + strand at 1325.105 kb on + strand at 1325.229 kb on + strand, within Shew_1151 at 1325.318 kb on + strand, within Shew_1151 at 1325.463 kb on + strand, within Shew_1151 at 1325.495 kb on + strand, within Shew_1151 at 1325.571 kb on - strand, within Shew_1151 at 1326.098 kb on + strand, within Shew_1152 at 1326.430 kb on + strand, within Shew_1152 at 1326.767 kb on - strand at 1327.098 kb on + strand, within Shew_1154 at 1327.133 kb on - strand, within Shew_1154 at 1327.464 kb on + strand, within Shew_1154
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 1,324,925 + +0.4 1,325,090 + +1.8 1,325,105 + +0.1 1,325,229 + Shew_1151 0.11 +0.4 1,325,318 + Shew_1151 0.24 +0.1 1,325,463 + Shew_1151 0.45 -0.2 1,325,495 + Shew_1151 0.49 +0.2 1,325,571 - Shew_1151 0.60 -1.2 1,326,098 + Shew_1152 0.26 +0.8 1,326,430 + Shew_1152 0.66 -2.6 1,326,767 - -0.7 1,327,098 + Shew_1154 0.21 +0.7 1,327,133 - Shew_1154 0.27 +0.8 1,327,464 + Shew_1154 0.77 +0.6
Or see this region's nucleotide sequence