Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1057 and Shew_1058 are separated by 44 nucleotides Shew_1058 and Shew_1059 are separated by 266 nucleotides Shew_1059 and Shew_1060 are separated by 66 nucleotides
Shew_1057: Shew_1057 - pyridoxal phosphate biosynthetic protein PdxJ (RefSeq), at 1,219,531 to 1,220,268
_1057
Shew_1058: Shew_1058 - holo-acyl-carrier-protein synthase (RefSeq), at 1,220,313 to 1,220,714
_1058
Shew_1059: Shew_1059 - hypothetical protein (RefSeq), at 1,220,981 to 1,221,523
_1059
Shew_1060: Shew_1060 - hypothetical protein (RefSeq), at 1,221,590 to 1,222,873
_1060
Position (kb)
1220
1221
1222 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 1220.302 kb on + strand at 1220.419 kb on - strand, within Shew_1058 at 1220.421 kb on + strand, within Shew_1058 at 1220.576 kb on + strand, within Shew_1058 at 1220.644 kb on + strand, within Shew_1058 at 1221.033 kb on - strand at 1221.293 kb on - strand, within Shew_1059 at 1221.431 kb on - strand, within Shew_1059 at 1221.491 kb on + strand at 1221.602 kb on + strand at 1221.635 kb on - strand at 1221.673 kb on + strand at 1221.713 kb on - strand at 1222.110 kb on + strand, within Shew_1060 at 1222.168 kb on + strand, within Shew_1060 at 1222.481 kb on - strand, within Shew_1060
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 1,220,302 + -0.2 1,220,419 - Shew_1058 0.26 +4.1 1,220,421 + Shew_1058 0.27 +4.3 1,220,576 + Shew_1058 0.65 +4.2 1,220,644 + Shew_1058 0.82 +3.8 1,221,033 - -0.1 1,221,293 - Shew_1059 0.57 -0.7 1,221,431 - Shew_1059 0.83 -1.2 1,221,491 + -0.4 1,221,602 + -0.7 1,221,635 - +0.1 1,221,673 + +0.1 1,221,713 - -0.4 1,222,110 + Shew_1060 0.40 +0.1 1,222,168 + Shew_1060 0.45 -0.1 1,222,481 - Shew_1060 0.69 +0.0
Or see this region's nucleotide sequence