Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1004 and Shew_1005 are separated by 32 nucleotides Shew_1005 and Shew_1006 are separated by 93 nucleotides Shew_1006 and Shew_1007 are separated by 297 nucleotides Shew_1007 and Shew_1008 are separated by 209 nucleotides
Shew_1004: Shew_1004 - undecaprenyl pyrophosphate phosphatase (RefSeq), at 1,161,251 to 1,162,051
_1004
Shew_1005: Shew_1005 - rRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 1,162,084 to 1,162,908
_1005
Shew_1006: Shew_1006 - ErfK/YbiS/YcfS/YnhG family protein (RefSeq), at 1,163,002 to 1,163,943
_1006
Shew_1007: Shew_1007 - major outer membrane lipoprotein, putative (RefSeq), at 1,164,241 to 1,164,507
_1007
Shew_1008: Shew_1008 - metal dependent phosphohydrolase (RefSeq), at 1,164,717 to 1,165,958
_1008
Position (kb)
1163
1164 Strain fitness (log2 ratio)
-2
-1
0
1 at 1162.025 kb on + strand at 1162.147 kb on + strand at 1162.155 kb on - strand at 1162.221 kb on + strand, within Shew_1005 at 1162.673 kb on + strand, within Shew_1005 at 1162.710 kb on + strand, within Shew_1005 at 1162.718 kb on - strand, within Shew_1005 at 1162.718 kb on - strand, within Shew_1005 at 1162.718 kb on - strand, within Shew_1005 at 1162.732 kb on - strand, within Shew_1005 at 1163.170 kb on + strand, within Shew_1006 at 1163.982 kb on - strand at 1164.079 kb on - strand at 1164.440 kb on - strand, within Shew_1007
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 1,162,025 + +0.9 1,162,147 + +0.3 1,162,155 - +0.4 1,162,221 + Shew_1005 0.17 -0.6 1,162,673 + Shew_1005 0.71 -0.2 1,162,710 + Shew_1005 0.76 -0.2 1,162,718 - Shew_1005 0.77 -0.4 1,162,718 - Shew_1005 0.77 -0.1 1,162,718 - Shew_1005 0.77 -0.7 1,162,732 - Shew_1005 0.79 -0.9 1,163,170 + Shew_1006 0.18 -0.0 1,163,982 - +0.5 1,164,079 - -0.1 1,164,440 - Shew_1007 0.75 -2.7
Or see this region's nucleotide sequence