Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0216 and Shew_0217 are separated by 74 nucleotides Shew_0217 and Shew_0218 are separated by 8 nucleotides Shew_0218 and Shew_0219 are separated by 211 nucleotides Shew_0219 and Shew_0220 are separated by 2 nucleotides
Shew_0216: Shew_0216 - DNA adenine methylase (RefSeq), at 231,996 to 232,835
_0216
Shew_0217: Shew_0217 - hypothetical protein (RefSeq), at 232,910 to 233,083
_0217
Shew_0218: Shew_0218 - hypothetical protein (RefSeq), at 233,092 to 233,436
_0218
Shew_0219: Shew_0219 - ribulose-phosphate 3-epimerase (RefSeq), at 233,648 to 234,322
_0219
Shew_0220: Shew_0220 - phosphoglycolate phosphatase (RefSeq), at 234,325 to 235,020
_0220
Position (kb) 233
234 Strain fitness (log2 ratio) -1
0
1 at 232.439 kb on + strand, within Shew_0216 at 232.497 kb on - strand, within Shew_0216 at 232.877 kb on + strand at 232.981 kb on + strand, within Shew_0217 at 233.086 kb on - strand at 233.147 kb on + strand, within Shew_0218 at 233.194 kb on + strand, within Shew_0218 at 233.370 kb on - strand, within Shew_0218 at 233.425 kb on - strand at 234.312 kb on - strand at 234.312 kb on - strand at 234.411 kb on + strand, within Shew_0220
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 232,439 + Shew_0216 0.53 -1.7 232,497 - Shew_0216 0.60 -1.7 232,877 + +0.3 232,981 + Shew_0217 0.41 +0.7 233,086 - +0.0 233,147 + Shew_0218 0.16 -0.8 233,194 + Shew_0218 0.30 -1.2 233,370 - Shew_0218 0.81 -1.0 233,425 - -0.0 234,312 - -1.1 234,312 - +0.1 234,411 + Shew_0220 0.12 +0.2
Or see this region's nucleotide sequence