Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0210 and Shew_0211 overlap by 4 nucleotides Shew_0211 and Shew_0212 are separated by 22 nucleotides Shew_0212 and Shew_0213 are separated by 215 nucleotides Shew_0213 and Shew_0214 are separated by 15 nucleotides
Shew_0210: Shew_0210 - pilus assembly protein, PilO (RefSeq), at 225,359 to 225,982
_0210
Shew_0211: Shew_0211 - pilus assembly protein, PilQ (RefSeq), at 225,979 to 226,494
_0211
Shew_0212: Shew_0212 - type IV pilus secretin PilQ (RefSeq), at 226,517 to 228,568
_0212
Shew_0213: Shew_0213 - shikimate kinase I (RefSeq), at 228,784 to 229,299
_0213
Shew_0214: Shew_0214 - 3-dehydroquinate synthase (RefSeq), at 229,315 to 230,391
_0214
Position (kb)
226
227
228
229 Strain fitness (log2 ratio)
-2
-1
0
1 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.702 kb on + strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.710 kb on - strand, within Shew_0210 at 225.825 kb on - strand, within Shew_0210 at 225.837 kb on - strand, within Shew_0210 at 226.204 kb on + strand, within Shew_0211 at 226.271 kb on + strand, within Shew_0211 at 226.289 kb on - strand, within Shew_0211 at 226.380 kb on - strand, within Shew_0211 at 226.480 kb on - strand at 226.480 kb on - strand at 226.572 kb on - strand at 226.572 kb on - strand at 226.620 kb on - strand at 226.628 kb on + strand at 226.640 kb on + strand at 226.821 kb on + strand, within Shew_0212 at 226.824 kb on - strand, within Shew_0212 at 226.844 kb on - strand, within Shew_0212 at 226.854 kb on - strand, within Shew_0212 at 226.873 kb on + strand, within Shew_0212 at 226.916 kb on - strand, within Shew_0212 at 227.076 kb on + strand, within Shew_0212 at 227.096 kb on - strand, within Shew_0212 at 227.141 kb on + strand, within Shew_0212 at 227.141 kb on + strand, within Shew_0212 at 227.358 kb on - strand, within Shew_0212 at 227.423 kb on + strand, within Shew_0212 at 227.499 kb on - strand, within Shew_0212 at 227.611 kb on + strand, within Shew_0212 at 227.619 kb on - strand, within Shew_0212 at 227.624 kb on - strand, within Shew_0212 at 227.668 kb on + strand, within Shew_0212 at 227.676 kb on - strand, within Shew_0212 at 227.676 kb on - strand, within Shew_0212 at 227.707 kb on - strand, within Shew_0212 at 227.829 kb on + strand, within Shew_0212 at 227.920 kb on - strand, within Shew_0212 at 227.938 kb on - strand, within Shew_0212 at 228.099 kb on - strand, within Shew_0212 at 228.256 kb on - strand, within Shew_0212 at 228.307 kb on + strand, within Shew_0212 at 228.315 kb on - strand, within Shew_0212 at 228.329 kb on - strand, within Shew_0212 at 228.366 kb on + strand at 228.381 kb on - strand at 228.552 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 225,702 + Shew_0210 0.55 -1.9 225,702 + Shew_0210 0.55 -0.2 225,702 + Shew_0210 0.55 -0.3 225,710 - Shew_0210 0.56 +0.4 225,710 - Shew_0210 0.56 +0.5 225,825 - Shew_0210 0.75 +0.4 225,837 - Shew_0210 0.77 +0.5 226,204 + Shew_0211 0.44 -0.0 226,271 + Shew_0211 0.57 -0.7 226,289 - Shew_0211 0.60 +1.1 226,380 - Shew_0211 0.78 +0.2 226,480 - +0.2 226,480 - -0.5 226,572 - -0.7 226,572 - -1.1 226,620 - +1.3 226,628 + +0.2 226,640 + +0.4 226,821 + Shew_0212 0.15 -0.0 226,824 - Shew_0212 0.15 -0.8 226,844 - Shew_0212 0.16 +0.0 226,854 - Shew_0212 0.16 -1.9 226,873 + Shew_0212 0.17 +0.6 226,916 - Shew_0212 0.19 +0.6 227,076 + Shew_0212 0.27 -0.6 227,096 - Shew_0212 0.28 +0.2 227,141 + Shew_0212 0.30 -0.1 227,141 + Shew_0212 0.30 -0.6 227,358 - Shew_0212 0.41 -0.5 227,423 + Shew_0212 0.44 -0.0 227,499 - Shew_0212 0.48 -0.3 227,611 + Shew_0212 0.53 -0.2 227,619 - Shew_0212 0.54 -1.1 227,624 - Shew_0212 0.54 +0.2 227,668 + Shew_0212 0.56 +1.2 227,676 - Shew_0212 0.56 -0.8 227,676 - Shew_0212 0.56 +0.1 227,707 - Shew_0212 0.58 +0.1 227,829 + Shew_0212 0.64 -0.3 227,920 - Shew_0212 0.68 -1.7 227,938 - Shew_0212 0.69 -0.2 228,099 - Shew_0212 0.77 +0.4 228,256 - Shew_0212 0.85 +0.1 228,307 + Shew_0212 0.87 +0.4 228,315 - Shew_0212 0.88 -1.9 228,329 - Shew_0212 0.88 +0.3 228,366 + -0.1 228,381 - +0.8 228,552 + +0.3
Or see this region's nucleotide sequence