Experiment: Cephalothin 0.05 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0080 and Shew_0081 are separated by 6 nucleotides Shew_0081 and Shew_0082 are separated by 52 nucleotides Shew_0082 and Shew_0083 are separated by 134 nucleotides
Shew_0080: Shew_0080 - integral membrane sensor signal transduction histidine kinase (RefSeq), at 93,574 to 94,866
_0080
Shew_0081: Shew_0081 - two component transcriptional regulator (RefSeq), at 94,873 to 95,607
_0081
Shew_0082: Shew_0082 - hypothetical protein (RefSeq), at 95,660 to 96,109
_0082
Shew_0083: Shew_0083 - MOSC domain-containing protein (RefSeq), at 96,244 to 96,936
_0083
Position (kb)
94
95
96 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 93.955 kb on - strand, within Shew_0080 at 94.003 kb on - strand, within Shew_0080 at 94.014 kb on - strand, within Shew_0080 at 94.054 kb on + strand, within Shew_0080 at 94.145 kb on - strand, within Shew_0080 at 94.160 kb on + strand, within Shew_0080 at 94.168 kb on - strand, within Shew_0080 at 94.283 kb on - strand, within Shew_0080 at 94.517 kb on - strand, within Shew_0080 at 94.517 kb on - strand, within Shew_0080 at 94.694 kb on + strand, within Shew_0080 at 94.785 kb on + strand at 94.802 kb on - strand at 94.843 kb on - strand at 95.002 kb on + strand, within Shew_0081 at 95.439 kb on + strand, within Shew_0081 at 95.887 kb on + strand, within Shew_0082 at 95.890 kb on - strand, within Shew_0082 at 95.948 kb on - strand, within Shew_0082 at 96.087 kb on - strand at 96.187 kb on - strand at 96.371 kb on - strand, within Shew_0083
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.05 mg/ml remove 93,955 - Shew_0080 0.29 +0.5 94,003 - Shew_0080 0.33 +0.0 94,014 - Shew_0080 0.34 -0.2 94,054 + Shew_0080 0.37 -0.4 94,145 - Shew_0080 0.44 -3.7 94,160 + Shew_0080 0.45 -1.1 94,168 - Shew_0080 0.46 -1.0 94,283 - Shew_0080 0.55 -0.9 94,517 - Shew_0080 0.73 -2.8 94,517 - Shew_0080 0.73 -1.6 94,694 + Shew_0080 0.87 -1.4 94,785 + +0.7 94,802 - -0.4 94,843 - -0.5 95,002 + Shew_0081 0.18 +0.2 95,439 + Shew_0081 0.77 +0.4 95,887 + Shew_0082 0.50 +0.3 95,890 - Shew_0082 0.51 -0.1 95,948 - Shew_0082 0.64 +0.1 96,087 - +0.0 96,187 - -0.1 96,371 - Shew_0083 0.18 -0.2
Or see this region's nucleotide sequence