Experiment: Nickel (II) chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO4393 and pspE-2 are separated by 132 nucleotides pspE-2 and SO4395 are separated by 39 nucleotides SO4395 and acpD are separated by 61 nucleotides acpD and SO4397 are separated by 134 nucleotides
SO4393: SO4393 - acetyltransferase, GNAT family (NCBI ptt file), at 4,589,077 to 4,589,532
SO4393
SO4394: pspE-2 - phage shock protein E (NCBI ptt file), at 4,589,665 to 4,589,991
pspE-2
SO4395: SO4395 - hypothetical protein (NCBI ptt file), at 4,590,031 to 4,590,195
SO4395
SO4396: acpD - acyl carrier protein phosphodiesterase (NCBI ptt file), at 4,590,257 to 4,590,853
acpD
SO4397: SO4397 - hypothetical acetyltransferase (NCBI ptt file), at 4,590,988 to 4,591,425
SO4397
Position (kb) 4589
4590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4588.882 kb on - strand at 4588.965 kb on + strand at 4588.970 kb on + strand at 4589.028 kb on - strand at 4589.180 kb on + strand, within SO4393 at 4589.188 kb on - strand, within SO4393 at 4589.203 kb on - strand, within SO4393 at 4589.235 kb on - strand, within SO4393 at 4589.445 kb on - strand, within SO4393 at 4589.552 kb on - strand at 4589.839 kb on - strand, within pspE-2 at 4589.839 kb on - strand, within pspE-2 at 4589.861 kb on + strand, within pspE-2 at 4589.869 kb on - strand, within pspE-2 at 4589.869 kb on - strand, within pspE-2 at 4589.979 kb on + strand at 4589.979 kb on + strand at 4589.979 kb on + strand at 4589.987 kb on - strand at 4590.022 kb on - strand at 4590.078 kb on + strand, within SO4395 at 4590.078 kb on + strand, within SO4395 at 4590.078 kb on + strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.086 kb on - strand, within SO4395 at 4590.124 kb on + strand, within SO4395 at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.289 kb on - strand at 4590.318 kb on + strand, within acpD at 4590.334 kb on - strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.339 kb on + strand, within acpD at 4590.404 kb on - strand, within acpD at 4590.508 kb on - strand, within acpD at 4590.511 kb on + strand, within acpD at 4590.514 kb on - strand, within acpD at 4590.589 kb on + strand, within acpD at 4590.592 kb on - strand, within acpD at 4590.597 kb on - strand, within acpD at 4590.624 kb on + strand, within acpD at 4590.664 kb on + strand, within acpD at 4590.672 kb on - strand, within acpD at 4590.677 kb on + strand, within acpD at 4590.742 kb on - strand, within acpD at 4590.912 kb on + strand at 4590.914 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM remove 4,588,882 - +0.3 4,588,965 + -0.6 4,588,970 + -0.9 4,589,028 - +1.0 4,589,180 + SO4393 0.23 -0.0 4,589,188 - SO4393 0.24 +0.8 4,589,203 - SO4393 0.28 -0.2 4,589,235 - SO4393 0.35 +1.3 4,589,445 - SO4393 0.81 -0.9 4,589,552 - -0.2 4,589,839 - pspE-2 SO4394 0.53 +0.3 4,589,839 - pspE-2 SO4394 0.53 +1.0 4,589,861 + pspE-2 SO4394 0.60 +0.1 4,589,869 - pspE-2 SO4394 0.62 +3.3 4,589,869 - pspE-2 SO4394 0.62 +0.6 4,589,979 + +0.3 4,589,979 + +0.2 4,589,979 + +1.8 4,589,987 - -0.7 4,590,022 - -0.7 4,590,078 + SO4395 0.28 -0.5 4,590,078 + SO4395 0.28 +0.5 4,590,078 + SO4395 0.28 +1.5 4,590,086 - SO4395 0.33 -0.3 4,590,086 - SO4395 0.33 +1.0 4,590,086 - SO4395 0.33 -0.5 4,590,124 + SO4395 0.56 +0.0 4,590,289 - +1.1 4,590,289 - +0.2 4,590,289 - -3.0 4,590,289 - -1.6 4,590,289 - -1.1 4,590,289 - -0.7 4,590,318 + acpD SO4396 0.10 -1.1 4,590,334 - acpD SO4396 0.13 +0.1 4,590,339 + acpD SO4396 0.14 -1.1 4,590,339 + acpD SO4396 0.14 -1.2 4,590,339 + acpD SO4396 0.14 +0.5 4,590,404 - acpD SO4396 0.25 -2.4 4,590,508 - acpD SO4396 0.42 +0.5 4,590,511 + acpD SO4396 0.43 -0.3 4,590,514 - acpD SO4396 0.43 +0.1 4,590,589 + acpD SO4396 0.56 -0.1 4,590,592 - acpD SO4396 0.56 +0.6 4,590,597 - acpD SO4396 0.57 +0.6 4,590,624 + acpD SO4396 0.61 +0.4 4,590,664 + acpD SO4396 0.68 +0.2 4,590,672 - acpD SO4396 0.70 -2.0 4,590,677 + acpD SO4396 0.70 -0.4 4,590,742 - acpD SO4396 0.81 -0.4 4,590,912 + +0.6 4,590,914 - +0.6
Or see this region's nucleotide sequence