Experiment: Nickel (II) chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SO3518 and glnB-2 are separated by 73 nucleotides glnB-2 and SO3520 are separated by 245 nucleotides
SO3518: SO3518 - ISSod4, transposase (NCBI ptt file), at 3,672,859 to 3,674,061
SO3518
SO3519: glnB-2 - nitrogen regulatory protein P-II 1 (NCBI ptt file), at 3,674,135 to 3,674,473
glnB-2
SO3520: SO3520 - type IV pilus biogenesis protein, putative (NCBI ptt file), at 3,674,719 to 3,675,231
SO3520
Position (kb)
3674
3675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3674.224 kb on - strand, within glnB-2 at 3674.316 kb on + strand, within glnB-2 at 3674.341 kb on + strand, within glnB-2 at 3674.344 kb on - strand, within glnB-2 at 3674.363 kb on + strand, within glnB-2 at 3674.395 kb on - strand, within glnB-2 at 3674.509 kb on + strand at 3674.517 kb on + strand at 3674.517 kb on + strand at 3674.517 kb on + strand at 3674.525 kb on - strand at 3674.531 kb on - strand at 3674.533 kb on + strand at 3674.533 kb on + strand at 3674.541 kb on - strand at 3674.679 kb on - strand at 3674.679 kb on - strand at 3674.679 kb on - strand at 3674.690 kb on + strand at 3674.704 kb on + strand at 3674.707 kb on - strand at 3674.723 kb on + strand at 3674.731 kb on - strand at 3674.731 kb on - strand at 3674.733 kb on - strand at 3674.738 kb on + strand at 3674.738 kb on + strand at 3674.738 kb on + strand at 3674.744 kb on + strand at 3674.746 kb on - strand at 3674.746 kb on - strand at 3674.752 kb on - strand at 3674.752 kb on - strand at 3674.752 kb on - strand at 3674.757 kb on + strand at 3674.757 kb on + strand at 3674.759 kb on + strand at 3674.764 kb on + strand at 3674.765 kb on - strand at 3674.785 kb on - strand, within SO3520 at 3674.813 kb on - strand, within SO3520 at 3674.827 kb on - strand, within SO3520 at 3674.829 kb on + strand, within SO3520 at 3674.829 kb on + strand, within SO3520 at 3674.837 kb on - strand, within SO3520 at 3674.837 kb on - strand, within SO3520 at 3674.896 kb on - strand, within SO3520 at 3674.911 kb on + strand, within SO3520 at 3674.930 kb on - strand, within SO3520 at 3674.935 kb on + strand, within SO3520 at 3674.943 kb on - strand, within SO3520 at 3674.982 kb on + strand, within SO3520 at 3674.990 kb on + strand, within SO3520 at 3675.019 kb on + strand, within SO3520 at 3675.021 kb on + strand, within SO3520 at 3675.029 kb on + strand, within SO3520 at 3675.111 kb on - strand, within SO3520 at 3675.159 kb on + strand, within SO3520 at 3675.161 kb on - strand, within SO3520 at 3675.169 kb on + strand, within SO3520 at 3675.193 kb on + strand at 3675.206 kb on - strand at 3675.206 kb on - strand at 3675.206 kb on - strand at 3675.252 kb on - strand at 3675.263 kb on + strand at 3675.265 kb on + strand at 3675.265 kb on + strand at 3675.271 kb on - strand at 3675.273 kb on + strand at 3675.273 kb on + strand at 3675.273 kb on - strand at 3675.275 kb on + strand at 3675.275 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.280 kb on + strand at 3675.283 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.288 kb on - strand at 3675.290 kb on + strand at 3675.296 kb on - strand at 3675.298 kb on - strand at 3675.298 kb on - strand at 3675.298 kb on - strand at 3675.300 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.308 kb on + strand at 3675.316 kb on + strand at 3675.316 kb on - strand at 3675.316 kb on - strand at 3675.316 kb on - strand at 3675.323 kb on - strand at 3675.330 kb on + strand at 3675.330 kb on + strand at 3675.330 kb on + strand at 3675.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM remove 3,674,224 - glnB-2 SO3519 0.26 -1.0 3,674,316 + glnB-2 SO3519 0.53 -1.3 3,674,341 + glnB-2 SO3519 0.61 -0.6 3,674,344 - glnB-2 SO3519 0.62 -1.4 3,674,363 + glnB-2 SO3519 0.67 -1.1 3,674,395 - glnB-2 SO3519 0.77 +0.9 3,674,509 + -1.4 3,674,517 + -1.5 3,674,517 + +0.4 3,674,517 + -1.1 3,674,525 - -0.1 3,674,531 - +0.9 3,674,533 + -0.7 3,674,533 + +0.7 3,674,541 - -0.8 3,674,679 - -0.4 3,674,679 - -0.2 3,674,679 - +2.2 3,674,690 + -0.8 3,674,704 + +0.2 3,674,707 - -0.1 3,674,723 + -1.1 3,674,731 - -0.3 3,674,731 - +0.7 3,674,733 - -1.0 3,674,738 + +0.8 3,674,738 + +0.2 3,674,738 + -1.2 3,674,744 + -0.3 3,674,746 - -0.5 3,674,746 - -2.8 3,674,752 - -0.4 3,674,752 - +2.3 3,674,752 - +0.2 3,674,757 + +0.4 3,674,757 + +1.3 3,674,759 + +0.5 3,674,764 + -1.2 3,674,765 - -0.1 3,674,785 - SO3520 0.13 -0.0 3,674,813 - SO3520 0.18 -0.2 3,674,827 - SO3520 0.21 -1.4 3,674,829 + SO3520 0.21 +0.7 3,674,829 + SO3520 0.21 +0.3 3,674,837 - SO3520 0.23 -0.5 3,674,837 - SO3520 0.23 -0.6 3,674,896 - SO3520 0.35 -2.1 3,674,911 + SO3520 0.37 -0.3 3,674,930 - SO3520 0.41 +0.5 3,674,935 + SO3520 0.42 +0.2 3,674,943 - SO3520 0.44 -0.0 3,674,982 + SO3520 0.51 -0.5 3,674,990 + SO3520 0.53 -0.5 3,675,019 + SO3520 0.58 +0.9 3,675,021 + SO3520 0.59 +0.1 3,675,029 + SO3520 0.60 +0.9 3,675,111 - SO3520 0.76 +0.5 3,675,159 + SO3520 0.86 +0.6 3,675,161 - SO3520 0.86 -0.3 3,675,169 + SO3520 0.88 -1.1 3,675,193 + -0.5 3,675,206 - +0.3 3,675,206 - +0.9 3,675,206 - +0.8 3,675,252 - -0.6 3,675,263 + -0.7 3,675,265 + +0.3 3,675,265 + -0.0 3,675,271 - +0.3 3,675,273 + -0.1 3,675,273 + +0.8 3,675,273 - -0.4 3,675,275 + +0.2 3,675,275 + -0.7 3,675,280 + -0.8 3,675,280 + -0.9 3,675,280 + +0.6 3,675,280 + -0.2 3,675,280 + -0.8 3,675,283 - -0.6 3,675,288 - -0.4 3,675,288 - -0.3 3,675,288 - -0.1 3,675,288 - +0.8 3,675,288 - -0.2 3,675,288 - +1.4 3,675,290 + +0.2 3,675,296 - -1.1 3,675,298 - +0.4 3,675,298 - -0.9 3,675,298 - -1.8 3,675,300 + -2.2 3,675,308 + -0.7 3,675,308 + +0.9 3,675,308 + +0.5 3,675,308 + -0.9 3,675,308 + -0.8 3,675,308 + +0.3 3,675,308 + -1.1 3,675,308 + -1.6 3,675,308 + -0.1 3,675,316 + -0.2 3,675,316 - +0.6 3,675,316 - +0.9 3,675,316 - -0.3 3,675,323 - -0.7 3,675,330 + +0.6 3,675,330 + -0.2 3,675,330 + +1.4 3,675,420 - -0.1
Or see this region's nucleotide sequence