Strain Fitness in Shewanella oneidensis MR-1 around SO3063

Experiment: Nickel (II) chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttopB and SO3062 are separated by 199 nucleotidesSO3062 and SO3063 are separated by 293 nucleotidesSO3063 and purF are separated by 95 nucleotides SO3061: topB - DNA topoisomerase III (NCBI ptt file), at 3,175,740 to 3,177,848 topB SO3062: SO3062 - hypothetical protein (NCBI ptt file), at 3,178,048 to 3,178,335 SO3062 SO3063: SO3063 - sodium:alanine symporter family protein (NCBI ptt file), at 3,178,629 to 3,180,116 SO3063 SO3064: purF - amidophosphoribosyltransferase (NCBI ptt file), at 3,180,212 to 3,181,726 purF Position (kb) 3178 3179 3180 3181Strain fitness (log2 ratio) -2 -1 0 1at 3177.735 kb on - strandat 3177.903 kb on - strandat 3177.969 kb on - strandat 3178.041 kb on - strandat 3178.151 kb on + strand, within SO3062at 3178.159 kb on - strand, within SO3062at 3178.247 kb on + strand, within SO3062at 3178.388 kb on + strandat 3178.411 kb on + strandat 3178.500 kb on + strandat 3178.531 kb on + strandat 3178.660 kb on - strandat 3178.818 kb on + strand, within SO3063at 3178.818 kb on + strand, within SO3063at 3178.831 kb on - strand, within SO3063at 3178.956 kb on + strand, within SO3063at 3178.962 kb on + strand, within SO3063at 3179.004 kb on + strand, within SO3063at 3179.103 kb on + strand, within SO3063at 3179.160 kb on - strand, within SO3063at 3179.261 kb on + strand, within SO3063at 3179.430 kb on + strand, within SO3063at 3179.430 kb on + strand, within SO3063at 3179.438 kb on - strand, within SO3063at 3179.466 kb on + strand, within SO3063at 3179.547 kb on + strand, within SO3063at 3179.580 kb on + strand, within SO3063at 3179.587 kb on + strand, within SO3063at 3179.595 kb on - strand, within SO3063at 3179.665 kb on + strand, within SO3063at 3179.690 kb on - strand, within SO3063at 3180.065 kb on + strandat 3180.065 kb on + strandat 3180.203 kb on + strandat 3180.236 kb on - strandat 3180.242 kb on - strandat 3180.293 kb on - strandat 3180.343 kb on - strandat 3180.669 kb on + strand, within purFat 3180.886 kb on - strand, within purFat 3180.897 kb on - strand, within purF

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM
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3,177,735 - +0.6
3,177,903 - +0.1
3,177,969 - -0.7
3,178,041 - +0.4
3,178,151 + SO3062 0.36 -1.2
3,178,159 - SO3062 0.39 -0.6
3,178,247 + SO3062 0.69 -0.6
3,178,388 + -0.0
3,178,411 + +1.1
3,178,500 + +0.6
3,178,531 + +0.1
3,178,660 - +1.2
3,178,818 + SO3063 0.13 +0.2
3,178,818 + SO3063 0.13 +0.2
3,178,831 - SO3063 0.14 -1.5
3,178,956 + SO3063 0.22 +1.0
3,178,962 + SO3063 0.22 +0.5
3,179,004 + SO3063 0.25 +1.5
3,179,103 + SO3063 0.32 +0.5
3,179,160 - SO3063 0.36 +0.9
3,179,261 + SO3063 0.42 +1.2
3,179,430 + SO3063 0.54 +0.7
3,179,430 + SO3063 0.54 +0.8
3,179,438 - SO3063 0.54 +0.2
3,179,466 + SO3063 0.56 -0.4
3,179,547 + SO3063 0.62 +0.2
3,179,580 + SO3063 0.64 +0.3
3,179,587 + SO3063 0.64 +1.1
3,179,595 - SO3063 0.65 +0.7
3,179,665 + SO3063 0.70 +0.1
3,179,690 - SO3063 0.71 +0.3
3,180,065 + +1.0
3,180,065 + +0.5
3,180,203 + -0.4
3,180,236 - -1.9
3,180,242 - +0.1
3,180,293 - +0.1
3,180,343 - -0.5
3,180,669 + purF SO3064 0.30 +0.2
3,180,886 - purF SO3064 0.44 -1.2
3,180,897 - purF SO3064 0.45 +0.5

Or see this region's nucleotide sequence