Experiment: Nickel (II) chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrA and hcp are separated by 216 nucleotides hcp and SO1364 are separated by 216 nucleotides
SO1362: tyrA - chorismate mutase/prephenate dehydrogenase (NCBI ptt file), at 1,416,836 to 1,417,975
tyrA
SO1363: hcp - prismane protein (NCBI ptt file), at 1,418,192 to 1,419,856
hcp
SO1364: SO1364 - iron-sulfur cluster-binding protein (NCBI ptt file), at 1,420,073 to 1,421,050
SO1364
Position (kb)
1418
1419
1420 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1417.304 kb on + strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.488 kb on - strand, within tyrA at 1417.628 kb on + strand, within tyrA at 1417.641 kb on + strand, within tyrA at 1417.641 kb on + strand, within tyrA at 1417.753 kb on + strand, within tyrA at 1417.829 kb on + strand, within tyrA at 1418.057 kb on - strand at 1418.128 kb on + strand at 1418.204 kb on - strand at 1418.241 kb on + strand at 1418.291 kb on + strand at 1418.465 kb on + strand, within hcp at 1418.465 kb on + strand, within hcp at 1418.465 kb on + strand, within hcp at 1418.597 kb on + strand, within hcp at 1418.664 kb on + strand, within hcp at 1418.899 kb on + strand, within hcp at 1418.907 kb on - strand, within hcp at 1418.958 kb on + strand, within hcp at 1418.966 kb on - strand, within hcp at 1419.074 kb on + strand, within hcp at 1419.092 kb on - strand, within hcp at 1419.211 kb on - strand, within hcp at 1419.213 kb on + strand, within hcp at 1419.214 kb on + strand, within hcp at 1419.222 kb on - strand, within hcp at 1419.226 kb on - strand, within hcp at 1419.226 kb on - strand, within hcp at 1419.236 kb on + strand, within hcp at 1419.394 kb on - strand, within hcp at 1419.571 kb on + strand, within hcp at 1419.571 kb on + strand, within hcp at 1419.571 kb on + strand, within hcp at 1419.662 kb on - strand, within hcp at 1419.662 kb on - strand, within hcp at 1419.762 kb on + strand at 1419.868 kb on + strand at 1419.911 kb on - strand at 1420.001 kb on + strand at 1420.001 kb on + strand at 1420.145 kb on + strand at 1420.174 kb on + strand, within SO1364 at 1420.174 kb on + strand, within SO1364 at 1420.174 kb on + strand, within SO1364 at 1420.190 kb on - strand, within SO1364 at 1420.251 kb on - strand, within SO1364 at 1420.295 kb on - strand, within SO1364 at 1420.374 kb on + strand, within SO1364 at 1420.374 kb on + strand, within SO1364 at 1420.382 kb on - strand, within SO1364 at 1420.382 kb on - strand, within SO1364 at 1420.503 kb on - strand, within SO1364 at 1420.632 kb on + strand, within SO1364 at 1420.680 kb on + strand, within SO1364 at 1420.696 kb on + strand, within SO1364 at 1420.743 kb on + strand, within SO1364 at 1420.743 kb on + strand, within SO1364 at 1420.788 kb on + strand, within SO1364 at 1420.802 kb on - strand, within SO1364 at 1420.825 kb on + strand, within SO1364 at 1420.833 kb on - strand, within SO1364
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM remove 1,417,304 + tyrA SO1362 0.41 +0.5 1,417,488 - tyrA SO1362 0.57 +0.1 1,417,488 - tyrA SO1362 0.57 -0.9 1,417,488 - tyrA SO1362 0.57 +0.4 1,417,628 + tyrA SO1362 0.69 +0.1 1,417,641 + tyrA SO1362 0.71 +0.9 1,417,641 + tyrA SO1362 0.71 -0.7 1,417,753 + tyrA SO1362 0.80 -0.8 1,417,829 + tyrA SO1362 0.87 -0.2 1,418,057 - +0.1 1,418,128 + -0.8 1,418,204 - +1.1 1,418,241 + +0.8 1,418,291 + +0.3 1,418,465 + hcp SO1363 0.16 +0.2 1,418,465 + hcp SO1363 0.16 -0.7 1,418,465 + hcp SO1363 0.16 -0.7 1,418,597 + hcp SO1363 0.24 -1.5 1,418,664 + hcp SO1363 0.28 -0.2 1,418,899 + hcp SO1363 0.42 +0.1 1,418,907 - hcp SO1363 0.43 -0.7 1,418,958 + hcp SO1363 0.46 -0.5 1,418,966 - hcp SO1363 0.46 -1.0 1,419,074 + hcp SO1363 0.53 +0.2 1,419,092 - hcp SO1363 0.54 -1.9 1,419,211 - hcp SO1363 0.61 +0.3 1,419,213 + hcp SO1363 0.61 -2.3 1,419,214 + hcp SO1363 0.61 -0.8 1,419,222 - hcp SO1363 0.62 -0.1 1,419,226 - hcp SO1363 0.62 +1.1 1,419,226 - hcp SO1363 0.62 -3.1 1,419,236 + hcp SO1363 0.63 +0.8 1,419,394 - hcp SO1363 0.72 +0.6 1,419,571 + hcp SO1363 0.83 +0.3 1,419,571 + hcp SO1363 0.83 +0.4 1,419,571 + hcp SO1363 0.83 -0.3 1,419,662 - hcp SO1363 0.88 -1.9 1,419,662 - hcp SO1363 0.88 +0.4 1,419,762 + +0.6 1,419,868 + +0.8 1,419,911 - -0.7 1,420,001 + -0.5 1,420,001 + -3.2 1,420,145 + +0.7 1,420,174 + SO1364 0.10 +0.3 1,420,174 + SO1364 0.10 -0.1 1,420,174 + SO1364 0.10 -0.7 1,420,190 - SO1364 0.12 -0.8 1,420,251 - SO1364 0.18 +1.2 1,420,295 - SO1364 0.23 +0.7 1,420,374 + SO1364 0.31 -1.5 1,420,374 + SO1364 0.31 -0.4 1,420,382 - SO1364 0.32 -0.4 1,420,382 - SO1364 0.32 +1.0 1,420,503 - SO1364 0.44 +0.5 1,420,632 + SO1364 0.57 +0.3 1,420,680 + SO1364 0.62 +0.2 1,420,696 + SO1364 0.64 +0.3 1,420,743 + SO1364 0.69 -0.5 1,420,743 + SO1364 0.69 +1.0 1,420,788 + SO1364 0.73 +0.2 1,420,802 - SO1364 0.75 +0.6 1,420,825 + SO1364 0.77 -0.9 1,420,833 - SO1364 0.78 -0.0
Or see this region's nucleotide sequence