Strain Fitness in Shewanella oneidensis MR-1 around SO1013

Experiment: Nickel (II) chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnuoL and nuoK overlap by 4 nucleotidesnuoK and nuoJ overlap by 4 nucleotidesnuoJ and nuoI are separated by 10 nucleotidesnuoI and nuoH are separated by 8 nucleotides SO1011: nuoL - NADH dehydrogenase I, L subunit (NCBI ptt file), at 1,048,384 to 1,050,231 nuoL SO1012: nuoK - NADH dehydrogenase I, K subunit (NCBI ptt file), at 1,050,228 to 1,050,536 nuoK SO1013: nuoJ - NADH dehydrogenase I, J subunit (NCBI ptt file), at 1,050,533 to 1,051,084 nuoJ SO1014: nuoI - NADH dehydrogenase I, I subunit (NCBI ptt file), at 1,051,095 to 1,051,637 nuoI SO1015: nuoH - NADH dehydrogenase I, H subunit (NCBI ptt file), at 1,051,646 to 1,052,614 nuoH Position (kb) 1050 1051 1052Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1049.581 kb on - strand, within nuoLat 1049.581 kb on - strand, within nuoLat 1049.583 kb on + strand, within nuoLat 1049.583 kb on + strand, within nuoLat 1049.583 kb on + strand, within nuoLat 1049.591 kb on - strand, within nuoLat 1049.637 kb on + strand, within nuoLat 1049.652 kb on + strand, within nuoLat 1049.697 kb on - strandat 1049.725 kb on - strand, within nuoLat 1049.725 kb on - strand, within nuoLat 1049.748 kb on + strand, within nuoLat 1049.774 kb on + strand, within nuoLat 1049.792 kb on - strand, within nuoLat 1050.114 kb on + strandat 1050.114 kb on + strandat 1050.122 kb on - strandat 1050.133 kb on + strandat 1050.178 kb on - strandat 1050.183 kb on - strandat 1050.261 kb on - strand, within nuoKat 1050.285 kb on - strand, within nuoKat 1050.315 kb on - strand, within nuoKat 1050.371 kb on - strand, within nuoKat 1050.459 kb on + strand, within nuoKat 1050.467 kb on - strand, within nuoKat 1050.481 kb on + strand, within nuoKat 1050.481 kb on + strand, within nuoKat 1050.503 kb on + strand, within nuoKat 1050.503 kb on + strand, within nuoKat 1050.511 kb on - strandat 1050.543 kb on + strandat 1050.587 kb on - strandat 1050.589 kb on + strand, within nuoJat 1050.605 kb on - strand, within nuoJat 1050.696 kb on + strand, within nuoJat 1050.706 kb on + strand, within nuoJat 1050.706 kb on + strand, within nuoJat 1050.714 kb on - strand, within nuoJat 1051.006 kb on + strand, within nuoJat 1051.155 kb on - strand, within nuoIat 1051.318 kb on + strand, within nuoIat 1051.446 kb on - strand, within nuoIat 1051.454 kb on - strand, within nuoIat 1051.485 kb on + strand, within nuoIat 1051.485 kb on + strand, within nuoIat 1051.493 kb on - strand, within nuoIat 1051.611 kb on + strandat 1051.677 kb on + strandat 1051.735 kb on - strandat 1051.746 kb on - strand, within nuoHat 1051.786 kb on + strand, within nuoHat 1051.786 kb on + strand, within nuoHat 1051.815 kb on - strand, within nuoHat 1051.869 kb on - strand, within nuoHat 1051.974 kb on + strand, within nuoHat 1051.996 kb on + strand, within nuoHat 1052.063 kb on + strand, within nuoHat 1052.063 kb on + strand, within nuoHat 1052.073 kb on + strand, within nuoH

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM
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1,049,581 - nuoL SO1011 0.65 -0.3
1,049,581 - nuoL SO1011 0.65 -1.7
1,049,583 + nuoL SO1011 0.65 -2.4
1,049,583 + nuoL SO1011 0.65 -0.7
1,049,583 + nuoL SO1011 0.65 +0.5
1,049,591 - nuoL SO1011 0.65 -0.6
1,049,637 + nuoL SO1011 0.68 -1.5
1,049,652 + nuoL SO1011 0.69 -1.1
1,049,697 - -0.3
1,049,725 - nuoL SO1011 0.73 -1.8
1,049,725 - nuoL SO1011 0.73 +0.2
1,049,748 + nuoL SO1011 0.74 -1.6
1,049,774 + nuoL SO1011 0.75 -1.2
1,049,792 - nuoL SO1011 0.76 -1.3
1,050,114 + -2.8
1,050,114 + +0.8
1,050,122 - -1.1
1,050,133 + -1.4
1,050,178 - -0.8
1,050,183 - -0.1
1,050,261 - nuoK SO1012 0.11 -0.5
1,050,285 - nuoK SO1012 0.18 -2.1
1,050,315 - nuoK SO1012 0.28 -1.6
1,050,371 - nuoK SO1012 0.46 -1.5
1,050,459 + nuoK SO1012 0.75 -0.7
1,050,467 - nuoK SO1012 0.77 +0.1
1,050,481 + nuoK SO1012 0.82 -0.6
1,050,481 + nuoK SO1012 0.82 -1.0
1,050,503 + nuoK SO1012 0.89 -0.1
1,050,503 + nuoK SO1012 0.89 -2.6
1,050,511 - -2.1
1,050,543 + -1.5
1,050,587 - -0.8
1,050,589 + nuoJ SO1013 0.10 -2.6
1,050,605 - nuoJ SO1013 0.13 -1.1
1,050,696 + nuoJ SO1013 0.30 -1.9
1,050,706 + nuoJ SO1013 0.31 -1.1
1,050,706 + nuoJ SO1013 0.31 -0.4
1,050,714 - nuoJ SO1013 0.33 -1.3
1,051,006 + nuoJ SO1013 0.86 -0.5
1,051,155 - nuoI SO1014 0.11 -0.7
1,051,318 + nuoI SO1014 0.41 -2.0
1,051,446 - nuoI SO1014 0.65 -0.1
1,051,454 - nuoI SO1014 0.66 -2.6
1,051,485 + nuoI SO1014 0.72 -1.2
1,051,485 + nuoI SO1014 0.72 -0.6
1,051,493 - nuoI SO1014 0.73 -1.1
1,051,611 + -0.3
1,051,677 + -0.6
1,051,735 - -1.1
1,051,746 - nuoH SO1015 0.10 -1.2
1,051,786 + nuoH SO1015 0.14 +0.5
1,051,786 + nuoH SO1015 0.14 -3.0
1,051,815 - nuoH SO1015 0.17 +0.5
1,051,869 - nuoH SO1015 0.23 -2.1
1,051,974 + nuoH SO1015 0.34 -0.4
1,051,996 + nuoH SO1015 0.36 -0.0
1,052,063 + nuoH SO1015 0.43 -4.1
1,052,063 + nuoH SO1015 0.43 -2.2
1,052,073 + nuoH SO1015 0.44 -0.2

Or see this region's nucleotide sequence