Strain Fitness in Shewanella oneidensis MR-1 around SO0177

Experiment: Nickel (II) chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgspM and gspN are separated by 14 nucleotidesgspN and SO0177 are separated by 59 nucleotidesSO0177 and SO0179 are separated by 151 nucleotidesSO0179 and SO0180 overlap by 23 nucleotides SO0175: gspM - general secretion pathway protein M (NCBI ptt file), at 181,623 to 182,099 gspM SO0176: gspN - general secretion pathway protein N (NCBI ptt file), at 182,114 to 182,875 gspN SO0177: SO0177 - HAD-superfamily hydrolase, subfamily IA, variant 3 protein family (NCBI ptt file), at 182,935 to 183,597 SO0177 SO0179: SO0179 - conserved hypothetical protein (NCBI ptt file), at 183,749 to 184,327 SO0179 SO0180: SO0180 - hypothetical protein (NCBI ptt file), at 184,305 to 186,314 SO0180 Position (kb) 182 183 184Strain fitness (log2 ratio) -3 -2 -1 0 1at 182.067 kb on + strandat 182.081 kb on + strandat 182.104 kb on + strandat 182.149 kb on + strandat 182.149 kb on + strandat 182.152 kb on - strandat 182.171 kb on + strandat 182.229 kb on + strand, within gspNat 182.319 kb on + strand, within gspNat 182.404 kb on - strand, within gspNat 182.420 kb on + strand, within gspNat 182.425 kb on - strand, within gspNat 182.548 kb on + strand, within gspNat 182.626 kb on + strand, within gspNat 182.637 kb on + strand, within gspNat 182.645 kb on - strand, within gspNat 182.722 kb on + strand, within gspNat 182.748 kb on - strand, within gspNat 182.758 kb on + strand, within gspNat 182.766 kb on - strand, within gspNat 182.787 kb on - strand, within gspNat 182.827 kb on + strandat 182.869 kb on + strandat 182.966 kb on - strandat 183.038 kb on - strand, within SO0177at 183.051 kb on - strand, within SO0177at 183.203 kb on - strand, within SO0177at 183.213 kb on - strand, within SO0177at 183.228 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.242 kb on + strand, within SO0177at 183.324 kb on - strand, within SO0177at 183.501 kb on + strand, within SO0177at 183.501 kb on + strand, within SO0177at 183.501 kb on + strand, within SO0177at 183.509 kb on - strand, within SO0177at 183.567 kb on + strandat 183.570 kb on - strandat 183.570 kb on - strandat 183.785 kb on - strandat 183.833 kb on - strand, within SO0179at 183.833 kb on - strand, within SO0179at 183.872 kb on + strand, within SO0179at 183.872 kb on - strand, within SO0179at 183.878 kb on + strand, within SO0179at 183.878 kb on + strand, within SO0179at 183.891 kb on - strand, within SO0179at 183.979 kb on - strand, within SO0179at 184.003 kb on - strand, within SO0179at 184.021 kb on + strand, within SO0179at 184.085 kb on + strand, within SO0179at 184.092 kb on + strand, within SO0179at 184.100 kb on - strand, within SO0179at 184.100 kb on - strand, within SO0179at 184.100 kb on - strand, within SO0179at 184.177 kb on + strand, within SO0179at 184.195 kb on - strand, within SO0179at 184.218 kb on + strand, within SO0179at 184.332 kb on + strandat 184.348 kb on + strandat 184.356 kb on - strandat 184.380 kb on + strandat 184.483 kb on + strandat 184.511 kb on - strand, within SO0180

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 0.4 mM
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182,067 + -1.1
182,081 + +0.3
182,104 + -0.5
182,149 + -2.3
182,149 + -2.2
182,152 - -1.3
182,171 + -1.3
182,229 + gspN SO0176 0.15 -1.3
182,319 + gspN SO0176 0.27 -3.1
182,404 - gspN SO0176 0.38 -2.5
182,420 + gspN SO0176 0.40 -2.8
182,425 - gspN SO0176 0.41 -1.4
182,548 + gspN SO0176 0.57 -2.3
182,626 + gspN SO0176 0.67 -2.6
182,637 + gspN SO0176 0.69 -3.7
182,645 - gspN SO0176 0.70 -1.8
182,722 + gspN SO0176 0.80 -0.4
182,748 - gspN SO0176 0.83 -1.0
182,758 + gspN SO0176 0.85 -1.2
182,766 - gspN SO0176 0.86 -2.0
182,787 - gspN SO0176 0.88 -1.7
182,827 + +0.1
182,869 + -0.0
182,966 - -0.1
183,038 - SO0177 0.16 -0.5
183,051 - SO0177 0.17 -0.0
183,203 - SO0177 0.40 -1.0
183,213 - SO0177 0.42 -0.2
183,228 + SO0177 0.44 +0.6
183,242 + SO0177 0.46 -1.5
183,242 + SO0177 0.46 -0.0
183,242 + SO0177 0.46 +0.3
183,242 + SO0177 0.46 -1.4
183,242 + SO0177 0.46 -0.6
183,242 + SO0177 0.46 +0.2
183,242 + SO0177 0.46 -0.9
183,242 + SO0177 0.46 -0.2
183,324 - SO0177 0.59 -0.0
183,501 + SO0177 0.85 -0.6
183,501 + SO0177 0.85 +0.1
183,501 + SO0177 0.85 -1.7
183,509 - SO0177 0.87 -2.1
183,567 + +0.2
183,570 - -0.3
183,570 - -1.2
183,785 - +0.4
183,833 - SO0179 0.15 +0.3
183,833 - SO0179 0.15 -0.4
183,872 + SO0179 0.21 -0.2
183,872 - SO0179 0.21 +0.4
183,878 + SO0179 0.22 -0.0
183,878 + SO0179 0.22 -0.3
183,891 - SO0179 0.25 -0.8
183,979 - SO0179 0.40 -0.4
184,003 - SO0179 0.44 -0.0
184,021 + SO0179 0.47 +1.0
184,085 + SO0179 0.58 +1.0
184,092 + SO0179 0.59 +0.9
184,100 - SO0179 0.61 -0.4
184,100 - SO0179 0.61 -1.2
184,100 - SO0179 0.61 -0.0
184,177 + SO0179 0.74 -0.9
184,195 - SO0179 0.77 +1.2
184,218 + SO0179 0.81 +0.5
184,332 + +0.2
184,348 + +0.0
184,356 - -1.1
184,380 + -0.4
184,483 + -0.2
184,511 - SO0180 0.10 -1.4

Or see this region's nucleotide sequence