Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sucC and Shewana3_1713 overlap by 1 nucleotides Shewana3_1713 and Shewana3_1714 are separated by 379 nucleotides Shewana3_1714 and Shewana3_1715 are separated by 31 nucleotides
Shewana3_1712: sucC - succinyl-CoA synthetase subunit beta (RefSeq), at 2,016,832 to 2,017,998
sucC
Shewana3_1713: Shewana3_1713 - succinyl-CoA synthetase (ADP-forming) alpha subunit (RefSeq), at 2,017,998 to 2,018,870
_1713
Shewana3_1714: Shewana3_1714 - GreA/GreB family elongation factor (RefSeq), at 2,019,250 to 2,019,657
_1714
Shewana3_1715: Shewana3_1715 - GCN5-related N-acetyltransferase (RefSeq), at 2,019,689 to 2,020,201
_1715
Position (kb)
2017
2018
2019 Strain fitness (log2 ratio)
-1
0
1
2 at 2017.453 kb on + strand, within sucC at 2017.864 kb on + strand, within sucC at 2018.052 kb on + strand at 2018.133 kb on + strand, within Shewana3_1713 at 2018.154 kb on + strand, within Shewana3_1713 at 2018.964 kb on + strand at 2018.972 kb on - strand at 2019.001 kb on + strand at 2019.070 kb on + strand at 2019.077 kb on + strand at 2019.078 kb on - strand at 2019.080 kb on - strand at 2019.151 kb on - strand at 2019.199 kb on - strand at 2019.204 kb on - strand at 2019.259 kb on - strand at 2019.496 kb on + strand, within Shewana3_1714 at 2019.523 kb on + strand, within Shewana3_1714 at 2019.665 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 2,017,453 + sucC Shewana3_1712 0.53 +0.5 2,017,864 + sucC Shewana3_1712 0.88 +1.9 2,018,052 + +0.1 2,018,133 + Shewana3_1713 0.15 +1.2 2,018,154 + Shewana3_1713 0.18 +1.3 2,018,964 + +0.6 2,018,972 - -0.2 2,019,001 + +0.1 2,019,070 + -0.3 2,019,077 + +0.2 2,019,078 - +0.7 2,019,080 - -1.1 2,019,151 - -0.6 2,019,199 - +0.3 2,019,204 - +1.0 2,019,259 - -0.4 2,019,496 + Shewana3_1714 0.60 -0.8 2,019,523 + Shewana3_1714 0.67 -0.7 2,019,665 + +0.2
Or see this region's nucleotide sequence