Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1335 and Shewana3_1336 are separated by 18 nucleotides Shewana3_1336 and fliS are separated by 6 nucleotides fliS and Shewana3_1338 are separated by 286 nucleotides
Shewana3_1335: Shewana3_1335 - flagellar hook-associated 2 domain-containing protein (RefSeq), at 1,547,975 to 1,549,345
_1335
Shewana3_1336: Shewana3_1336 - hypothetical protein (RefSeq), at 1,549,364 to 1,549,693
_1336
Shewana3_1337: fliS - flagellar protein FliS (RefSeq), at 1,549,700 to 1,550,113
fliS
Shewana3_1338: Shewana3_1338 - sigma-54 dependent trancsriptional regulator (RefSeq), at 1,550,400 to 1,551,833
_1338
Position (kb)
1549
1550
1551 Strain fitness (log2 ratio)
-1
0
1
2 at 1548.794 kb on + strand, within Shewana3_1335 at 1548.794 kb on + strand, within Shewana3_1335 at 1549.254 kb on + strand at 1549.298 kb on - strand at 1549.321 kb on + strand at 1549.512 kb on + strand, within Shewana3_1336 at 1549.562 kb on + strand, within Shewana3_1336 at 1549.571 kb on - strand, within Shewana3_1336 at 1549.694 kb on + strand at 1549.742 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.811 kb on + strand, within fliS at 1549.819 kb on - strand, within fliS at 1550.052 kb on + strand, within fliS at 1550.143 kb on + strand at 1550.204 kb on - strand at 1550.280 kb on + strand at 1550.298 kb on - strand at 1550.305 kb on - strand at 1550.698 kb on + strand, within Shewana3_1338 at 1550.706 kb on - strand, within Shewana3_1338 at 1550.879 kb on - strand, within Shewana3_1338
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 1,548,794 + Shewana3_1335 0.60 +0.2 1,548,794 + Shewana3_1335 0.60 +1.8 1,549,254 + +1.0 1,549,298 - +0.6 1,549,321 + +0.7 1,549,512 + Shewana3_1336 0.45 +0.1 1,549,562 + Shewana3_1336 0.60 +0.2 1,549,571 - Shewana3_1336 0.63 +0.2 1,549,694 + -0.7 1,549,742 + fliS Shewana3_1337 0.10 +1.2 1,549,811 + fliS Shewana3_1337 0.27 +1.8 1,549,811 + fliS Shewana3_1337 0.27 +0.4 1,549,811 + fliS Shewana3_1337 0.27 +1.4 1,549,819 - fliS Shewana3_1337 0.29 +1.1 1,550,052 + fliS Shewana3_1337 0.85 -0.0 1,550,143 + +0.2 1,550,204 - +0.8 1,550,280 + +1.6 1,550,298 - +1.9 1,550,305 - +1.7 1,550,698 + Shewana3_1338 0.21 +2.0 1,550,706 - Shewana3_1338 0.21 +1.1 1,550,879 - Shewana3_1338 0.33 +1.6
Or see this region's nucleotide sequence