Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1320 and flgB are separated by 144 nucleotides flgB and flgC are separated by 2 nucleotides flgC and flgD are separated by 24 nucleotides flgD and flgE are separated by 69 nucleotides
Shewana3_1320: Shewana3_1320 - MCP methyltransferase, CheR-type (RefSeq), at 1,533,497 to 1,534,336
_1320
Shewana3_1321: flgB - flagellar basal body rod protein FlgB (RefSeq), at 1,534,481 to 1,534,885
flgB
Shewana3_1322: flgC - flagellar basal body rod protein FlgC (RefSeq), at 1,534,888 to 1,535,304
flgC
Shewana3_1323: flgD - flagellar basal body rod modification protein (RefSeq), at 1,535,329 to 1,536,108
flgD
Shewana3_1324: flgE - flagellar hook protein FlgE (RefSeq), at 1,536,178 to 1,537,539
flgE
Position (kb)
1535
1536
1537 Strain fitness (log2 ratio)
-1
0
1
2 at 1534.509 kb on - strand at 1534.630 kb on - strand, within flgB at 1534.825 kb on - strand, within flgB at 1535.096 kb on + strand, within flgC at 1535.231 kb on + strand, within flgC at 1535.267 kb on + strand at 1535.482 kb on + strand, within flgD at 1535.490 kb on + strand, within flgD at 1535.490 kb on + strand, within flgD at 1535.490 kb on - strand, within flgD at 1535.498 kb on - strand, within flgD at 1535.769 kb on - strand, within flgD at 1535.854 kb on - strand, within flgD at 1535.861 kb on + strand, within flgD at 1536.068 kb on + strand at 1536.076 kb on - strand at 1536.223 kb on + strand at 1536.255 kb on + strand at 1536.271 kb on + strand at 1536.737 kb on + strand, within flgE at 1536.744 kb on - strand, within flgE at 1536.902 kb on + strand, within flgE at 1536.983 kb on - strand, within flgE at 1536.985 kb on + strand, within flgE
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 1,534,509 - +1.6 1,534,630 - flgB Shewana3_1321 0.37 +2.2 1,534,825 - flgB Shewana3_1321 0.85 +0.3 1,535,096 + flgC Shewana3_1322 0.50 +1.4 1,535,231 + flgC Shewana3_1322 0.82 +1.4 1,535,267 + +1.6 1,535,482 + flgD Shewana3_1323 0.20 +1.4 1,535,490 + flgD Shewana3_1323 0.21 +1.2 1,535,490 + flgD Shewana3_1323 0.21 +1.9 1,535,490 - flgD Shewana3_1323 0.21 +0.6 1,535,498 - flgD Shewana3_1323 0.22 +1.8 1,535,769 - flgD Shewana3_1323 0.56 +1.3 1,535,854 - flgD Shewana3_1323 0.67 +1.6 1,535,861 + flgD Shewana3_1323 0.68 +1.3 1,536,068 + +0.8 1,536,076 - +1.2 1,536,223 + +1.7 1,536,255 + -0.1 1,536,271 + +1.0 1,536,737 + flgE Shewana3_1324 0.41 +2.1 1,536,744 - flgE Shewana3_1324 0.42 +1.7 1,536,902 + flgE Shewana3_1324 0.53 +1.7 1,536,983 - flgE Shewana3_1324 0.59 +2.0 1,536,985 + flgE Shewana3_1324 0.59 +1.0
Or see this region's nucleotide sequence