Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0600 and Shewana3_0601 are separated by 368 nucleotides Shewana3_0601 and Shewana3_0602 overlap by 1 nucleotides Shewana3_0602 and Shewana3_0603 overlap by 1 nucleotides Shewana3_0603 and sspA are separated by 96 nucleotides
Shewana3_0600: Shewana3_0600 - HflC protein (RefSeq), at 703,661 to 704,554
_0600
Shewana3_0601: Shewana3_0601 - ubiquinol-cytochrome c reductase, iron-sulfur subunit (RefSeq), at 704,923 to 705,513
_0601
Shewana3_0602: Shewana3_0602 - cytochrome b/b6 domain-containing protein (RefSeq), at 705,513 to 706,727
_0602
Shewana3_0603: Shewana3_0603 - cytochrome c1 (RefSeq), at 706,727 to 707,425
_0603
Shewana3_0604: sspA - stringent starvation protein A (RefSeq), at 707,522 to 708,151
sspA
Position (kb) 705
706
707 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 704.829 kb on - strand at 705.718 kb on + strand, within Shewana3_0602 at 705.723 kb on + strand, within Shewana3_0602 at 706.009 kb on + strand, within Shewana3_0602 at 706.099 kb on - strand, within Shewana3_0602 at 706.396 kb on - strand, within Shewana3_0602 at 706.489 kb on + strand, within Shewana3_0602 at 706.594 kb on + strand, within Shewana3_0602 at 707.094 kb on + strand, within Shewana3_0603 at 707.202 kb on + strand, within Shewana3_0603 at 707.414 kb on + strand at 707.506 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 704,829 - -1.2 705,718 + Shewana3_0602 0.17 -2.0 705,723 + Shewana3_0602 0.17 -0.5 706,009 + Shewana3_0602 0.41 -2.6 706,099 - Shewana3_0602 0.48 -1.3 706,396 - Shewana3_0602 0.73 -1.4 706,489 + Shewana3_0602 0.80 -3.3 706,594 + Shewana3_0602 0.89 -0.3 707,094 + Shewana3_0603 0.53 -0.9 707,202 + Shewana3_0603 0.68 -2.1 707,414 + -0.1 707,506 + +4.1
Or see this region's nucleotide sequence