Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0599 and Shewana3_0600 are separated by 3 nucleotides Shewana3_0600 and Shewana3_0601 are separated by 368 nucleotides Shewana3_0601 and Shewana3_0602 overlap by 1 nucleotides
Shewana3_0599: Shewana3_0599 - HflK protein (RefSeq), at 702,512 to 703,657
_0599
Shewana3_0600: Shewana3_0600 - HflC protein (RefSeq), at 703,661 to 704,554
_0600
Shewana3_0601: Shewana3_0601 - ubiquinol-cytochrome c reductase, iron-sulfur subunit (RefSeq), at 704,923 to 705,513
_0601
Shewana3_0602: Shewana3_0602 - cytochrome b/b6 domain-containing protein (RefSeq), at 705,513 to 706,727
_0602
Position (kb) 703
704
705 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 702.777 kb on + strand, within Shewana3_0599 at 702.955 kb on + strand, within Shewana3_0599 at 702.992 kb on + strand, within Shewana3_0599 at 703.432 kb on + strand, within Shewana3_0599 at 703.546 kb on + strand at 703.546 kb on + strand at 703.604 kb on + strand at 704.209 kb on + strand, within Shewana3_0600 at 704.263 kb on + strand, within Shewana3_0600 at 704.481 kb on + strand at 704.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 702,777 + Shewana3_0599 0.23 +4.5 702,955 + Shewana3_0599 0.39 +3.6 702,992 + Shewana3_0599 0.42 +5.5 703,432 + Shewana3_0599 0.80 +4.5 703,546 + +5.2 703,546 + +4.0 703,604 + +3.2 704,209 + Shewana3_0600 0.61 +2.4 704,263 + Shewana3_0600 0.67 +1.0 704,481 + +2.1 704,829 - -1.2
Or see this region's nucleotide sequence