Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0415 and coaE overlap by 8 nucleotides coaE and Shewana3_0417 are separated by 93 nucleotides Shewana3_0417 and Shewana3_0418 are separated by 61 nucleotides
Shewana3_0415: Shewana3_0415 - hypothetical protein (RefSeq), at 473,002 to 473,736
_0415
Shewana3_0416: coaE - dephospho-CoA kinase (RefSeq), at 473,729 to 474,346
coaE
Shewana3_0417: Shewana3_0417 - type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A (RefSeq), at 474,440 to 475,369
_0417
Shewana3_0418: Shewana3_0418 - type II secretion system protein (RefSeq), at 475,431 to 476,702
_0418
Position (kb) 474
475
476 Strain fitness (log2 ratio) -1
0
1
2
3 at 473.651 kb on + strand, within Shewana3_0415 at 473.705 kb on + strand at 473.715 kb on + strand at 474.637 kb on - strand, within Shewana3_0417 at 474.999 kb on + strand, within Shewana3_0417 at 475.035 kb on - strand, within Shewana3_0417 at 475.133 kb on - strand, within Shewana3_0417 at 475.340 kb on + strand at 475.348 kb on - strand at 475.427 kb on - strand at 475.791 kb on + strand, within Shewana3_0418 at 476.070 kb on + strand, within Shewana3_0418 at 476.091 kb on + strand, within Shewana3_0418 at 476.202 kb on + strand, within Shewana3_0418 at 476.224 kb on + strand, within Shewana3_0418 at 476.229 kb on + strand, within Shewana3_0418
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 473,651 + Shewana3_0415 0.88 +0.6 473,705 + +0.4 473,715 + +0.1 474,637 - Shewana3_0417 0.21 +2.4 474,999 + Shewana3_0417 0.60 +3.4 475,035 - Shewana3_0417 0.64 +1.7 475,133 - Shewana3_0417 0.75 +1.9 475,340 + +0.8 475,348 - +2.3 475,427 - -0.0 475,791 + Shewana3_0418 0.28 +0.2 476,070 + Shewana3_0418 0.50 -0.1 476,091 + Shewana3_0418 0.52 +0.6 476,202 + Shewana3_0418 0.61 +0.1 476,224 + Shewana3_0418 0.62 +0.8 476,229 + Shewana3_0418 0.63 -0.1
Or see this region's nucleotide sequence