Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0397 and fis are separated by 254 nucleotides fis and Shewana3_0399 are separated by 16 nucleotides Shewana3_0399 and prmA are separated by 174 nucleotides
Shewana3_0397: Shewana3_0397 - hemerythrin HHE cation binding domain-containing protein (RefSeq), at 456,503 to 457,249
_0397
Shewana3_0398: fis - DNA-binding protein Fis (RefSeq), at 457,504 to 457,809
fis
Shewana3_0399: Shewana3_0399 - nifR3 family TIM-barrel protein (RefSeq), at 457,826 to 458,794
_0399
Shewana3_0400: prmA - ribosomal protein L11 methyltransferase (RefSeq), at 458,969 to 459,850
prmA
Position (kb) 457
458
459 Strain fitness (log2 ratio) -1
0
1
2
3 at 457.058 kb on - strand, within Shewana3_0397 at 457.111 kb on - strand, within Shewana3_0397 at 457.177 kb on + strand at 457.193 kb on + strand at 457.235 kb on - strand at 457.395 kb on - strand at 457.547 kb on - strand, within fis at 458.191 kb on - strand, within Shewana3_0399 at 458.368 kb on - strand, within Shewana3_0399 at 458.463 kb on - strand, within Shewana3_0399 at 458.543 kb on - strand, within Shewana3_0399 at 458.692 kb on - strand, within Shewana3_0399 at 458.789 kb on - strand at 458.819 kb on + strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.822 kb on - strand at 458.968 kb on - strand at 459.077 kb on - strand, within prmA at 459.143 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.390 kb on - strand, within prmA at 459.576 kb on + strand, within prmA at 459.666 kb on + strand, within prmA at 459.751 kb on + strand, within prmA at 459.759 kb on - strand, within prmA at 459.759 kb on - strand, within prmA
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 457,058 - Shewana3_0397 0.74 -0.6 457,111 - Shewana3_0397 0.81 +0.3 457,177 + +0.2 457,193 + -0.6 457,235 - +0.3 457,395 - +0.3 457,547 - fis Shewana3_0398 0.14 +0.9 458,191 - Shewana3_0399 0.38 +2.5 458,368 - Shewana3_0399 0.56 +1.1 458,463 - Shewana3_0399 0.66 +2.7 458,543 - Shewana3_0399 0.74 +1.3 458,692 - Shewana3_0399 0.89 +2.6 458,789 - -0.0 458,819 + +2.7 458,822 - +0.5 458,822 - +3.2 458,822 - +2.2 458,968 - -0.2 459,077 - prmA Shewana3_0400 0.12 -0.4 459,143 - prmA Shewana3_0400 0.20 -0.0 459,390 - prmA Shewana3_0400 0.48 +0.1 459,390 - prmA Shewana3_0400 0.48 +0.3 459,390 - prmA Shewana3_0400 0.48 -0.1 459,576 + prmA Shewana3_0400 0.69 +1.2 459,666 + prmA Shewana3_0400 0.79 +0.2 459,751 + prmA Shewana3_0400 0.89 +1.5 459,759 - prmA Shewana3_0400 0.90 -0.2 459,759 - prmA Shewana3_0400 0.90 +0.7
Or see this region's nucleotide sequence