Experiment: L-Alanine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0004 and trmE are separated by 103 nucleotides trmE and Shewana3_0006 are separated by 103 nucleotides
Shewana3_0004: Shewana3_0004 - TGF-beta receptor, type I/II extracellular region (RefSeq), at 3,725 to 5,272
_0004
Shewana3_0005: trmE - tRNA modification GTPase TrmE (RefSeq), at 5,376 to 6,737
trmE
Shewana3_0006: Shewana3_0006 - protein translocase subunit yidC (RefSeq), at 6,841 to 8,466
_0006
Position (kb) 5
6
7 Strain fitness (log2 ratio) -2
-1
0
1 at 4.438 kb on - strand, within Shewana3_0004 at 4.479 kb on - strand, within Shewana3_0004 at 4.492 kb on - strand, within Shewana3_0004 at 4.512 kb on + strand, within Shewana3_0004 at 4.578 kb on - strand, within Shewana3_0004 at 4.619 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.719 kb on - strand, within Shewana3_0004 at 4.786 kb on + strand, within Shewana3_0004 at 4.816 kb on + strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.851 kb on - strand, within Shewana3_0004 at 4.957 kb on - strand, within Shewana3_0004 at 5.160 kb on + strand at 5.231 kb on + strand at 5.433 kb on - strand at 5.536 kb on - strand, within trmE at 5.557 kb on + strand, within trmE at 5.610 kb on - strand, within trmE at 6.126 kb on + strand, within trmE at 6.126 kb on + strand, within trmE at 6.129 kb on - strand at 6.357 kb on + strand, within trmE at 6.569 kb on - strand, within trmE at 6.604 kb on + strand at 6.612 kb on + strand at 6.725 kb on + strand at 6.745 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (C) remove 4,438 - Shewana3_0004 0.46 +0.3 4,479 - Shewana3_0004 0.49 -0.2 4,492 - Shewana3_0004 0.50 +1.1 4,512 + Shewana3_0004 0.51 +0.1 4,578 - Shewana3_0004 0.55 -1.8 4,619 - Shewana3_0004 0.58 -0.1 4,719 - Shewana3_0004 0.64 +0.8 4,719 - Shewana3_0004 0.64 -1.3 4,786 + Shewana3_0004 0.69 -0.5 4,816 + Shewana3_0004 0.70 +0.1 4,851 - Shewana3_0004 0.73 -0.1 4,851 - Shewana3_0004 0.73 +0.3 4,957 - Shewana3_0004 0.80 +1.0 5,160 + -0.3 5,231 + +0.1 5,433 - -0.7 5,536 - trmE Shewana3_0005 0.12 +0.2 5,557 + trmE Shewana3_0005 0.13 -1.6 5,610 - trmE Shewana3_0005 0.17 -0.1 6,126 + trmE Shewana3_0005 0.55 +0.9 6,126 + trmE Shewana3_0005 0.55 +0.9 6,129 - +0.9 6,357 + trmE Shewana3_0005 0.72 -1.2 6,569 - trmE Shewana3_0005 0.88 -0.1 6,604 + +0.9 6,612 + -1.0 6,725 + -0.7 6,745 - +0.2
Or see this region's nucleotide sequence