Experiment: Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2102 and def are separated by 301 nucleotides def and Shew_2104 are separated by 179 nucleotides Shew_2104 and Shew_2105 are separated by 379 nucleotides Shew_2105 and Shew_2106 are separated by 48 nucleotides
Shew_2102: Shew_2102 - hypothetical protein (RefSeq), at 2,433,502 to 2,434,332
_2102
Shew_2103: def - peptide deformylase (RefSeq), at 2,434,634 to 2,435,125
def
Shew_2104: Shew_2104 - major facilitator transporter (RefSeq), at 2,435,305 to 2,437,026
_2104
Shew_2105: Shew_2105 - methylated-DNA-(protein)-cysteine S-methyltransferase (RefSeq), at 2,437,406 to 2,437,756
_2105
Shew_2106: Shew_2106 - hypothetical protein (RefSeq), at 2,437,805 to 2,438,596
_2106
Position (kb)
2435
2436
2437
2438 Strain fitness (log2 ratio)
-1
0
1 at 2434.794 kb on + strand, within def at 2434.800 kb on + strand, within def at 2434.975 kb on + strand, within def at 2435.331 kb on + strand at 2435.334 kb on - strand at 2435.339 kb on - strand at 2435.428 kb on + strand at 2435.591 kb on + strand, within Shew_2104 at 2435.710 kb on - strand, within Shew_2104 at 2436.036 kb on + strand, within Shew_2104 at 2436.036 kb on + strand, within Shew_2104 at 2436.124 kb on - strand, within Shew_2104 at 2436.430 kb on - strand, within Shew_2104 at 2436.461 kb on + strand, within Shew_2104 at 2436.469 kb on - strand, within Shew_2104 at 2436.521 kb on - strand, within Shew_2104 at 2436.906 kb on - strand at 2436.906 kb on - strand at 2436.947 kb on + strand at 2437.697 kb on - strand, within Shew_2105 at 2437.750 kb on - strand at 2437.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.08 mM remove 2,434,794 + def Shew_2103 0.33 -0.2 2,434,800 + def Shew_2103 0.34 -0.3 2,434,975 + def Shew_2103 0.69 +0.4 2,435,331 + +0.2 2,435,334 - -0.6 2,435,339 - +1.3 2,435,428 + -0.3 2,435,591 + Shew_2104 0.17 +1.0 2,435,710 - Shew_2104 0.24 +0.4 2,436,036 + Shew_2104 0.42 +0.3 2,436,036 + Shew_2104 0.42 +0.1 2,436,124 - Shew_2104 0.48 +0.2 2,436,430 - Shew_2104 0.65 -0.3 2,436,461 + Shew_2104 0.67 -0.4 2,436,469 - Shew_2104 0.68 -0.5 2,436,521 - Shew_2104 0.71 +0.1 2,436,906 - -0.1 2,436,906 - -1.3 2,436,947 + +0.0 2,437,697 - Shew_2105 0.83 +1.4 2,437,750 - -0.4 2,437,832 - -0.6
Or see this region's nucleotide sequence