Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_42 and HP15_43 are separated by 93 nucleotides HP15_43 and HP15_44 are separated by 179 nucleotides HP15_44 and HP15_45 are separated by 71 nucleotides
HP15_42: HP15_42 - short-chain dehydrogenase/reductase SDR, at 46,197 to 46,997
_42
HP15_43: HP15_43 - crotonobetaine/carnitine-CoA ligase, at 47,091 to 48,722
_43
HP15_44: HP15_44 - DitK protein, at 48,902 to 49,600
_44
HP15_45: HP15_45 - hypothetical protein, at 49,672 to 49,779
_45
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 46.211 kb on + strand at 46.258 kb on + strand at 46.327 kb on - strand, within HP15_42 at 46.341 kb on + strand, within HP15_42 at 46.431 kb on + strand, within HP15_42 at 46.541 kb on + strand, within HP15_42 at 46.541 kb on + strand, within HP15_42 at 46.596 kb on + strand, within HP15_42 at 46.596 kb on + strand, within HP15_42 at 46.596 kb on + strand, within HP15_42 at 46.645 kb on - strand, within HP15_42 at 46.773 kb on - strand, within HP15_42 at 46.796 kb on - strand, within HP15_42 at 46.938 kb on + strand at 47.027 kb on - strand at 47.070 kb on + strand at 47.155 kb on + strand at 47.243 kb on + strand at 47.285 kb on + strand, within HP15_43 at 47.293 kb on - strand, within HP15_43 at 47.293 kb on - strand, within HP15_43 at 47.311 kb on - strand, within HP15_43 at 47.323 kb on - strand, within HP15_43 at 47.325 kb on + strand, within HP15_43 at 47.325 kb on + strand, within HP15_43 at 47.333 kb on - strand, within HP15_43 at 47.488 kb on - strand, within HP15_43 at 47.529 kb on + strand, within HP15_43 at 47.535 kb on - strand, within HP15_43 at 47.537 kb on - strand, within HP15_43 at 47.559 kb on - strand, within HP15_43 at 47.573 kb on + strand, within HP15_43 at 47.600 kb on - strand, within HP15_43 at 47.685 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.809 kb on + strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.817 kb on - strand, within HP15_43 at 47.830 kb on + strand, within HP15_43 at 47.906 kb on + strand, within HP15_43 at 47.909 kb on - strand, within HP15_43 at 47.929 kb on - strand, within HP15_43 at 48.002 kb on - strand, within HP15_43 at 48.022 kb on - strand, within HP15_43 at 48.022 kb on - strand, within HP15_43 at 48.061 kb on - strand, within HP15_43 at 48.109 kb on + strand, within HP15_43 at 48.152 kb on + strand, within HP15_43 at 48.152 kb on + strand, within HP15_43 at 48.160 kb on - strand, within HP15_43 at 48.183 kb on + strand, within HP15_43 at 48.191 kb on - strand, within HP15_43 at 48.341 kb on - strand, within HP15_43 at 48.459 kb on - strand, within HP15_43 at 48.485 kb on - strand, within HP15_43 at 48.575 kb on - strand at 48.657 kb on - strand at 48.662 kb on - strand at 48.662 kb on - strand at 48.681 kb on - strand at 48.876 kb on + strand at 48.889 kb on + strand at 48.892 kb on - strand at 48.959 kb on - strand at 48.971 kb on - strand at 48.979 kb on + strand, within HP15_44 at 48.999 kb on - strand, within HP15_44 at 49.047 kb on + strand, within HP15_44 at 49.279 kb on - strand, within HP15_44 at 49.342 kb on - strand, within HP15_44 at 49.385 kb on - strand, within HP15_44 at 49.542 kb on + strand at 49.569 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 46,211 + +0.2 46,258 + +1.5 46,327 - HP15_42 0.16 -0.5 46,341 + HP15_42 0.18 -0.1 46,431 + HP15_42 0.29 -0.6 46,541 + HP15_42 0.43 -0.7 46,541 + HP15_42 0.43 -0.7 46,596 + HP15_42 0.50 -0.1 46,596 + HP15_42 0.50 -0.4 46,596 + HP15_42 0.50 -0.4 46,645 - HP15_42 0.56 -1.4 46,773 - HP15_42 0.72 -0.8 46,796 - HP15_42 0.75 -0.1 46,938 + -1.1 47,027 - -1.3 47,070 + +0.4 47,155 + -1.3 47,243 + -0.8 47,285 + HP15_43 0.12 -0.6 47,293 - HP15_43 0.12 +1.7 47,293 - HP15_43 0.12 +1.7 47,311 - HP15_43 0.13 +1.8 47,323 - HP15_43 0.14 +1.7 47,325 + HP15_43 0.14 +1.0 47,325 + HP15_43 0.14 +0.1 47,333 - HP15_43 0.15 +1.9 47,488 - HP15_43 0.24 +1.6 47,529 + HP15_43 0.27 -1.5 47,535 - HP15_43 0.27 -0.3 47,537 - HP15_43 0.27 +2.0 47,559 - HP15_43 0.29 +0.7 47,573 + HP15_43 0.30 +2.3 47,600 - HP15_43 0.31 -0.5 47,685 + HP15_43 0.36 -0.5 47,809 + HP15_43 0.44 -0.3 47,809 + HP15_43 0.44 +1.4 47,809 + HP15_43 0.44 -0.8 47,809 + HP15_43 0.44 -0.2 47,809 + HP15_43 0.44 +0.2 47,809 + HP15_43 0.44 +2.1 47,809 + HP15_43 0.44 +2.0 47,809 + HP15_43 0.44 +1.6 47,809 + HP15_43 0.44 -0.6 47,809 + HP15_43 0.44 +2.0 47,817 - HP15_43 0.44 +1.9 47,817 - HP15_43 0.44 +0.0 47,817 - HP15_43 0.44 +2.2 47,817 - HP15_43 0.44 -0.3 47,817 - HP15_43 0.44 -1.2 47,817 - HP15_43 0.44 +0.2 47,830 + HP15_43 0.45 +1.1 47,906 + HP15_43 0.50 -0.1 47,909 - HP15_43 0.50 -0.7 47,929 - HP15_43 0.51 -1.5 48,002 - HP15_43 0.56 +1.6 48,022 - HP15_43 0.57 +1.1 48,022 - HP15_43 0.57 +1.9 48,061 - HP15_43 0.59 -0.2 48,109 + HP15_43 0.62 +1.9 48,152 + HP15_43 0.65 +0.1 48,152 + HP15_43 0.65 +2.3 48,160 - HP15_43 0.66 +0.7 48,183 + HP15_43 0.67 +0.9 48,191 - HP15_43 0.67 +2.1 48,341 - HP15_43 0.77 +1.1 48,459 - HP15_43 0.84 -0.9 48,485 - HP15_43 0.85 +1.3 48,575 - +1.9 48,657 - +1.9 48,662 - +2.0 48,662 - -0.6 48,681 - +2.9 48,876 + -0.9 48,889 + -1.0 48,892 - -0.7 48,959 - +1.5 48,971 - -0.8 48,979 + HP15_44 0.11 -0.9 48,999 - HP15_44 0.14 -0.6 49,047 + HP15_44 0.21 +2.6 49,279 - HP15_44 0.54 -2.1 49,342 - HP15_44 0.63 +1.3 49,385 - HP15_44 0.69 +0.5 49,542 + -0.4 49,569 - -0.9
Or see this region's nucleotide sequence