Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_408 and HP15_409 are separated by 459 nucleotides HP15_409 and HP15_410 are separated by 83 nucleotides HP15_410 and HP15_411 overlap by 1 nucleotides
HP15_408: HP15_408 - single-stranded DNA-binding protein, at 404,357 to 404,881
_408
HP15_409: HP15_409 - hypothetical biogenesis protein MshI-like protein, at 405,341 to 406,048
_409
HP15_410: HP15_410 - fimbrial assembly family protein, at 406,132 to 406,650
_410
HP15_411: HP15_411 - MshA biogenesis protein, MshJ-like protein, at 406,650 to 407,339
_411
Position (kb) 405
406
407 Strain fitness (log2 ratio) -1
0
1
2
3 at 404.343 kb on + strand at 405.149 kb on + strand at 405.163 kb on + strand at 405.171 kb on - strand at 405.171 kb on - strand at 405.240 kb on - strand at 405.332 kb on - strand at 405.337 kb on - strand at 405.356 kb on - strand at 405.356 kb on - strand at 405.412 kb on - strand, within HP15_409 at 405.465 kb on - strand, within HP15_409 at 405.528 kb on - strand, within HP15_409 at 405.663 kb on + strand, within HP15_409 at 405.754 kb on - strand, within HP15_409 at 405.756 kb on + strand, within HP15_409 at 405.756 kb on + strand, within HP15_409 at 405.864 kb on - strand, within HP15_409 at 405.980 kb on + strand at 405.998 kb on - strand at 405.998 kb on - strand at 406.070 kb on - strand at 406.300 kb on + strand, within HP15_410 at 406.308 kb on - strand, within HP15_410 at 406.310 kb on + strand, within HP15_410 at 406.310 kb on + strand, within HP15_410 at 406.315 kb on + strand, within HP15_410 at 406.318 kb on - strand, within HP15_410 at 406.323 kb on - strand, within HP15_410 at 406.462 kb on + strand, within HP15_410 at 406.591 kb on + strand, within HP15_410 at 406.677 kb on - strand at 406.693 kb on + strand at 406.693 kb on + strand at 406.693 kb on + strand at 406.747 kb on + strand, within HP15_411 at 406.840 kb on - strand, within HP15_411 at 406.977 kb on + strand, within HP15_411 at 406.978 kb on - strand, within HP15_411 at 407.012 kb on + strand, within HP15_411 at 407.020 kb on - strand, within HP15_411
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 404,343 + -1.0 405,149 + +0.6 405,163 + +2.0 405,171 - +3.0 405,171 - +0.1 405,240 - +2.3 405,332 - +2.0 405,337 - +0.9 405,356 - -0.2 405,356 - +0.6 405,412 - HP15_409 0.10 +1.9 405,465 - HP15_409 0.18 +0.1 405,528 - HP15_409 0.26 +0.8 405,663 + HP15_409 0.45 +1.9 405,754 - HP15_409 0.58 +1.3 405,756 + HP15_409 0.59 +1.4 405,756 + HP15_409 0.59 +2.1 405,864 - HP15_409 0.74 +0.4 405,980 + +0.4 405,998 - +1.8 405,998 - +0.3 406,070 - -0.2 406,300 + HP15_410 0.32 +1.0 406,308 - HP15_410 0.34 +0.2 406,310 + HP15_410 0.34 +2.0 406,310 + HP15_410 0.34 +0.5 406,315 + HP15_410 0.35 +1.9 406,318 - HP15_410 0.36 +0.3 406,323 - HP15_410 0.37 +1.2 406,462 + HP15_410 0.64 +1.9 406,591 + HP15_410 0.88 +2.7 406,677 - +0.4 406,693 + +1.8 406,693 + +1.6 406,693 + +2.2 406,747 + HP15_411 0.14 +0.2 406,840 - HP15_411 0.28 -0.1 406,977 + HP15_411 0.47 +0.6 406,978 - HP15_411 0.48 -0.3 407,012 + HP15_411 0.52 -1.2 407,020 - HP15_411 0.54 +0.2
Or see this region's nucleotide sequence