Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4100 and HP15_4101 are separated by 11 nucleotides HP15_4101 and HP15_4102 are separated by 94 nucleotides HP15_4102 and HP15_4103 are separated by 94 nucleotides HP15_4103 and HP15_4104 are separated by 40 nucleotides
HP15_4100: HP15_4100 - long-chain-fatty-acid-CoA ligase, at 4,334,273 to 4,336,084
_4100
HP15_4101: HP15_4101 - membrane protein, at 4,336,096 to 4,336,416
_4101
HP15_4102: HP15_4102 - conserved hypothetical protein, at 4,336,511 to 4,337,497
_4102
HP15_4103: HP15_4103 - conserved hypothetical protein, at 4,337,592 to 4,338,239
_4103
HP15_4104: HP15_4104 - conserved hypothetical protein, at 4,338,280 to 4,338,672
_4104
Position (kb)
4336
4337
4338 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4335.687 kb on - strand, within HP15_4100 at 4335.687 kb on - strand, within HP15_4100 at 4335.738 kb on + strand, within HP15_4100 at 4335.810 kb on - strand, within HP15_4100 at 4335.834 kb on - strand, within HP15_4100 at 4335.839 kb on + strand, within HP15_4100 at 4335.922 kb on - strand at 4335.983 kb on + strand at 4336.013 kb on - strand at 4336.159 kb on - strand, within HP15_4101 at 4336.159 kb on - strand, within HP15_4101 at 4336.159 kb on - strand, within HP15_4101 at 4336.159 kb on - strand, within HP15_4101 at 4336.159 kb on - strand, within HP15_4101 at 4336.159 kb on - strand, within HP15_4101 at 4336.311 kb on - strand, within HP15_4101 at 4336.361 kb on + strand, within HP15_4101 at 4336.445 kb on - strand at 4336.494 kb on + strand at 4336.523 kb on + strand at 4336.523 kb on + strand at 4336.531 kb on - strand at 4336.619 kb on + strand, within HP15_4102 at 4336.655 kb on - strand, within HP15_4102 at 4336.675 kb on - strand, within HP15_4102 at 4336.781 kb on - strand, within HP15_4102 at 4336.870 kb on - strand, within HP15_4102 at 4336.886 kb on + strand, within HP15_4102 at 4337.023 kb on - strand, within HP15_4102 at 4337.055 kb on - strand, within HP15_4102 at 4337.071 kb on - strand, within HP15_4102 at 4337.110 kb on - strand, within HP15_4102 at 4337.113 kb on + strand, within HP15_4102 at 4337.138 kb on + strand, within HP15_4102 at 4337.222 kb on - strand, within HP15_4102 at 4337.310 kb on - strand, within HP15_4102 at 4337.315 kb on - strand, within HP15_4102 at 4337.315 kb on - strand, within HP15_4102 at 4337.391 kb on + strand, within HP15_4102 at 4337.406 kb on - strand at 4337.425 kb on - strand at 4337.430 kb on - strand at 4337.546 kb on + strand at 4337.554 kb on - strand at 4337.633 kb on + strand at 4337.651 kb on - strand at 4337.748 kb on + strand, within HP15_4103 at 4337.793 kb on + strand, within HP15_4103 at 4337.801 kb on - strand, within HP15_4103 at 4338.061 kb on + strand, within HP15_4103 at 4338.069 kb on - strand, within HP15_4103 at 4338.101 kb on + strand, within HP15_4103 at 4338.126 kb on - strand, within HP15_4103 at 4338.146 kb on - strand, within HP15_4103 at 4338.146 kb on - strand, within HP15_4103 at 4338.160 kb on + strand, within HP15_4103 at 4338.224 kb on - strand at 4338.248 kb on - strand at 4338.258 kb on - strand at 4338.258 kb on - strand at 4338.432 kb on - strand, within HP15_4104 at 4338.447 kb on - strand, within HP15_4104
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 4,335,687 - HP15_4100 0.78 +1.4 4,335,687 - HP15_4100 0.78 +2.3 4,335,738 + HP15_4100 0.81 +1.1 4,335,810 - HP15_4100 0.85 -0.6 4,335,834 - HP15_4100 0.86 +0.5 4,335,839 + HP15_4100 0.86 +0.4 4,335,922 - -0.3 4,335,983 + +0.0 4,336,013 - -0.2 4,336,159 - HP15_4101 0.20 -0.6 4,336,159 - HP15_4101 0.20 +0.3 4,336,159 - HP15_4101 0.20 -0.3 4,336,159 - HP15_4101 0.20 +1.7 4,336,159 - HP15_4101 0.20 -0.6 4,336,159 - HP15_4101 0.20 -0.3 4,336,311 - HP15_4101 0.67 -0.6 4,336,361 + HP15_4101 0.83 +0.1 4,336,445 - -0.6 4,336,494 + -1.3 4,336,523 + +1.5 4,336,523 + -0.1 4,336,531 - -0.8 4,336,619 + HP15_4102 0.11 -1.1 4,336,655 - HP15_4102 0.15 -1.2 4,336,675 - HP15_4102 0.17 -1.4 4,336,781 - HP15_4102 0.27 -1.3 4,336,870 - HP15_4102 0.36 -0.6 4,336,886 + HP15_4102 0.38 -0.8 4,337,023 - HP15_4102 0.52 +0.2 4,337,055 - HP15_4102 0.55 -0.5 4,337,071 - HP15_4102 0.57 +0.1 4,337,110 - HP15_4102 0.61 +0.0 4,337,113 + HP15_4102 0.61 +0.0 4,337,138 + HP15_4102 0.64 +0.6 4,337,222 - HP15_4102 0.72 +1.9 4,337,310 - HP15_4102 0.81 +2.1 4,337,315 - HP15_4102 0.81 -1.4 4,337,315 - HP15_4102 0.81 +1.5 4,337,391 + HP15_4102 0.89 -1.3 4,337,406 - +1.9 4,337,425 - +1.7 4,337,430 - +2.2 4,337,546 + -1.6 4,337,554 - +0.5 4,337,633 + -1.2 4,337,651 - +1.6 4,337,748 + HP15_4103 0.24 +0.1 4,337,793 + HP15_4103 0.31 -0.3 4,337,801 - HP15_4103 0.32 -0.5 4,338,061 + HP15_4103 0.72 -0.6 4,338,069 - HP15_4103 0.74 +1.7 4,338,101 + HP15_4103 0.79 -0.4 4,338,126 - HP15_4103 0.82 -0.6 4,338,146 - HP15_4103 0.85 -0.4 4,338,146 - HP15_4103 0.85 -1.9 4,338,160 + HP15_4103 0.88 -0.1 4,338,224 - -1.3 4,338,248 - +1.7 4,338,258 - -2.1 4,338,258 - +0.4 4,338,432 - HP15_4104 0.39 -0.9 4,338,447 - HP15_4104 0.42 -1.1
Or see this region's nucleotide sequence