Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4097 and HP15_4098 are separated by 71 nucleotides HP15_4098 and HP15_4099 are separated by 8 nucleotides HP15_4099 and HP15_4100 overlap by 13 nucleotides
HP15_4097: HP15_4097 - inner-membrane translocator, at 4,331,144 to 4,332,187
_4097
HP15_4098: HP15_4098 - amino acids binding protein, at 4,332,259 to 4,333,467
_4098
HP15_4099: HP15_4099 - branched-chain amino acid ABC transporter, ATP-binding protein, at 4,333,476 to 4,334,285
_4099
HP15_4100: HP15_4100 - long-chain-fatty-acid-CoA ligase, at 4,334,273 to 4,336,084
_4100
Position (kb)
4332
4333
4334 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4331.311 kb on - strand, within HP15_4097 at 4331.329 kb on - strand, within HP15_4097 at 4331.442 kb on + strand, within HP15_4097 at 4331.450 kb on - strand, within HP15_4097 at 4331.458 kb on + strand, within HP15_4097 at 4331.489 kb on + strand, within HP15_4097 at 4331.514 kb on + strand, within HP15_4097 at 4331.518 kb on + strand, within HP15_4097 at 4331.612 kb on - strand, within HP15_4097 at 4331.695 kb on + strand, within HP15_4097 at 4331.697 kb on - strand, within HP15_4097 at 4331.792 kb on - strand, within HP15_4097 at 4331.805 kb on - strand, within HP15_4097 at 4331.834 kb on - strand, within HP15_4097 at 4331.893 kb on - strand, within HP15_4097 at 4331.945 kb on + strand, within HP15_4097 at 4332.225 kb on - strand at 4332.319 kb on + strand at 4332.327 kb on - strand at 4332.332 kb on - strand at 4332.354 kb on - strand at 4332.354 kb on - strand at 4332.421 kb on - strand, within HP15_4098 at 4332.443 kb on + strand, within HP15_4098 at 4332.453 kb on + strand, within HP15_4098 at 4332.627 kb on - strand, within HP15_4098 at 4332.643 kb on + strand, within HP15_4098 at 4332.643 kb on + strand, within HP15_4098 at 4332.665 kb on + strand, within HP15_4098 at 4332.846 kb on + strand, within HP15_4098 at 4332.854 kb on - strand, within HP15_4098 at 4333.021 kb on + strand, within HP15_4098 at 4333.245 kb on + strand, within HP15_4098 at 4333.245 kb on + strand, within HP15_4098 at 4333.258 kb on - strand, within HP15_4098 at 4333.271 kb on + strand, within HP15_4098 at 4333.315 kb on + strand, within HP15_4098 at 4333.315 kb on + strand, within HP15_4098 at 4333.320 kb on + strand, within HP15_4098 at 4333.323 kb on - strand, within HP15_4098 at 4333.323 kb on - strand, within HP15_4098 at 4333.333 kb on - strand, within HP15_4098 at 4333.333 kb on - strand, within HP15_4098 at 4333.333 kb on - strand, within HP15_4098 at 4333.420 kb on + strand at 4333.449 kb on - strand at 4333.476 kb on + strand at 4333.512 kb on + strand at 4333.520 kb on - strand at 4333.551 kb on - strand at 4333.624 kb on - strand, within HP15_4099 at 4333.666 kb on - strand, within HP15_4099 at 4333.740 kb on - strand, within HP15_4099 at 4333.780 kb on - strand, within HP15_4099 at 4333.873 kb on + strand, within HP15_4099 at 4333.943 kb on - strand, within HP15_4099 at 4334.052 kb on + strand, within HP15_4099 at 4334.070 kb on + strand, within HP15_4099 at 4334.089 kb on - strand, within HP15_4099 at 4334.126 kb on - strand, within HP15_4099 at 4334.279 kb on + strand at 4334.331 kb on - strand at 4334.392 kb on + strand at 4334.400 kb on - strand at 4334.439 kb on - strand at 4334.444 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 4,331,311 - HP15_4097 0.16 -0.5 4,331,329 - HP15_4097 0.18 -1.0 4,331,442 + HP15_4097 0.29 +0.7 4,331,450 - HP15_4097 0.29 -0.1 4,331,458 + HP15_4097 0.30 -0.1 4,331,489 + HP15_4097 0.33 +0.7 4,331,514 + HP15_4097 0.35 +0.4 4,331,518 + HP15_4097 0.36 +1.0 4,331,612 - HP15_4097 0.45 +1.6 4,331,695 + HP15_4097 0.53 -0.3 4,331,697 - HP15_4097 0.53 -0.3 4,331,792 - HP15_4097 0.62 +1.2 4,331,805 - HP15_4097 0.63 +1.5 4,331,834 - HP15_4097 0.66 +2.6 4,331,893 - HP15_4097 0.72 +1.6 4,331,945 + HP15_4097 0.77 -1.3 4,332,225 - -0.5 4,332,319 + +2.7 4,332,327 - -0.3 4,332,332 - -0.8 4,332,354 - -1.4 4,332,354 - -1.1 4,332,421 - HP15_4098 0.13 -0.1 4,332,443 + HP15_4098 0.15 -0.2 4,332,453 + HP15_4098 0.16 +1.1 4,332,627 - HP15_4098 0.30 +2.3 4,332,643 + HP15_4098 0.32 -1.7 4,332,643 + HP15_4098 0.32 +2.0 4,332,665 + HP15_4098 0.34 +1.1 4,332,846 + HP15_4098 0.49 -0.3 4,332,854 - HP15_4098 0.49 -1.2 4,333,021 + HP15_4098 0.63 +1.8 4,333,245 + HP15_4098 0.82 +0.1 4,333,245 + HP15_4098 0.82 +0.4 4,333,258 - HP15_4098 0.83 +1.8 4,333,271 + HP15_4098 0.84 +1.2 4,333,315 + HP15_4098 0.87 +0.3 4,333,315 + HP15_4098 0.87 +2.1 4,333,320 + HP15_4098 0.88 -0.3 4,333,323 - HP15_4098 0.88 -0.3 4,333,323 - HP15_4098 0.88 -0.6 4,333,333 - HP15_4098 0.89 -0.8 4,333,333 - HP15_4098 0.89 +2.1 4,333,333 - HP15_4098 0.89 -1.5 4,333,420 + -0.6 4,333,449 - +1.0 4,333,476 + +1.2 4,333,512 + -2.1 4,333,520 - -0.9 4,333,551 - -0.9 4,333,624 - HP15_4099 0.18 -0.2 4,333,666 - HP15_4099 0.23 -0.5 4,333,740 - HP15_4099 0.33 +0.1 4,333,780 - HP15_4099 0.38 -0.6 4,333,873 + HP15_4099 0.49 -0.7 4,333,943 - HP15_4099 0.58 +1.7 4,334,052 + HP15_4099 0.71 +0.0 4,334,070 + HP15_4099 0.73 +1.9 4,334,089 - HP15_4099 0.76 +0.9 4,334,126 - HP15_4099 0.80 -0.2 4,334,279 + +0.0 4,334,331 - -0.2 4,334,392 + +0.8 4,334,400 - -0.3 4,334,439 - +2.0 4,334,444 - -1.2
Or see this region's nucleotide sequence