Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_39 and HP15_40 are separated by 430 nucleotides HP15_40 and HP15_41 are separated by 156 nucleotides HP15_41 and HP15_42 are separated by 478 nucleotides
HP15_39: HP15_39 - ring hydroxylating dioxygenase, alpha subunit, at 43,300 to 44,616
_39
HP15_40: HP15_40 - dioxygenase DitA oxygenase component small subunit-like protein, at 45,047 to 45,211
_40
HP15_41: HP15_41 - conserved hypothetical protein, at 45,368 to 45,718
_41
HP15_42: HP15_42 - short-chain dehydrogenase/reductase SDR, at 46,197 to 46,997
_42
Position (kb) 45
46 Strain fitness (log2 ratio) -2
-1
0
1
2 at 44.091 kb on + strand, within HP15_39 at 44.152 kb on - strand, within HP15_39 at 44.164 kb on + strand, within HP15_39 at 44.165 kb on - strand, within HP15_39 at 44.179 kb on - strand, within HP15_39 at 44.214 kb on + strand, within HP15_39 at 44.254 kb on - strand, within HP15_39 at 44.274 kb on + strand, within HP15_39 at 44.276 kb on + strand, within HP15_39 at 44.282 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.284 kb on - strand, within HP15_39 at 44.377 kb on + strand, within HP15_39 at 44.401 kb on - strand, within HP15_39 at 44.412 kb on + strand, within HP15_39 at 44.412 kb on + strand, within HP15_39 at 44.420 kb on - strand, within HP15_39 at 44.434 kb on + strand, within HP15_39 at 44.604 kb on - strand at 44.611 kb on + strand at 44.633 kb on + strand at 44.661 kb on - strand at 44.709 kb on + strand at 44.709 kb on + strand at 44.709 kb on + strand at 44.716 kb on - strand at 44.717 kb on - strand at 44.807 kb on + strand at 44.825 kb on + strand at 44.887 kb on - strand at 44.904 kb on + strand at 44.909 kb on + strand at 44.912 kb on - strand at 45.091 kb on - strand, within HP15_40 at 45.101 kb on - strand, within HP15_40 at 45.142 kb on - strand, within HP15_40 at 45.143 kb on - strand, within HP15_40 at 45.308 kb on - strand at 45.473 kb on - strand, within HP15_41 at 45.568 kb on + strand, within HP15_41 at 45.577 kb on - strand, within HP15_41 at 45.587 kb on - strand, within HP15_41 at 45.645 kb on + strand, within HP15_41 at 45.653 kb on - strand, within HP15_41 at 45.668 kb on - strand, within HP15_41 at 45.668 kb on - strand, within HP15_41 at 45.675 kb on - strand, within HP15_41 at 45.765 kb on + strand at 45.773 kb on + strand at 45.802 kb on - strand at 45.858 kb on - strand at 45.869 kb on + strand at 45.963 kb on + strand at 45.995 kb on + strand at 46.003 kb on - strand at 46.003 kb on - strand at 46.046 kb on - strand at 46.062 kb on + strand at 46.211 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 44,091 + HP15_39 0.60 -0.5 44,152 - HP15_39 0.65 +1.5 44,164 + HP15_39 0.66 +1.7 44,165 - HP15_39 0.66 +0.8 44,179 - HP15_39 0.67 -1.6 44,214 + HP15_39 0.69 -1.3 44,254 - HP15_39 0.72 +2.1 44,274 + HP15_39 0.74 -1.7 44,276 + HP15_39 0.74 +1.2 44,282 - HP15_39 0.75 -0.1 44,284 - HP15_39 0.75 +0.2 44,284 - HP15_39 0.75 -0.5 44,284 - HP15_39 0.75 -0.9 44,284 - HP15_39 0.75 -1.7 44,377 + HP15_39 0.82 -0.9 44,401 - HP15_39 0.84 +1.6 44,412 + HP15_39 0.84 +1.5 44,412 + HP15_39 0.84 -0.8 44,420 - HP15_39 0.85 -1.8 44,434 + HP15_39 0.86 -0.5 44,604 - +2.3 44,611 + +1.5 44,633 + -1.2 44,661 - +1.8 44,709 + +1.7 44,709 + -0.4 44,709 + -0.6 44,716 - -0.3 44,717 - +1.7 44,807 + +2.4 44,825 + +2.1 44,887 - -0.9 44,904 + +1.5 44,909 + -1.4 44,912 - +1.6 45,091 - HP15_40 0.27 +0.5 45,101 - HP15_40 0.33 -1.2 45,142 - HP15_40 0.58 -1.4 45,143 - HP15_40 0.58 -0.6 45,308 - -1.3 45,473 - HP15_41 0.30 +0.2 45,568 + HP15_41 0.57 +0.1 45,577 - HP15_41 0.60 -0.2 45,587 - HP15_41 0.62 +0.6 45,645 + HP15_41 0.79 +0.1 45,653 - HP15_41 0.81 +1.4 45,668 - HP15_41 0.85 +2.0 45,668 - HP15_41 0.85 -1.0 45,675 - HP15_41 0.87 -0.7 45,765 + -0.5 45,773 + +2.3 45,802 - +1.5 45,858 - -0.9 45,869 + -0.8 45,963 + +0.7 45,995 + -0.9 46,003 - +1.7 46,003 - +1.8 46,046 - -0.9 46,062 + +0.7 46,211 + +0.2
Or see this region's nucleotide sequence