Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_256 and HP15_257 are separated by 6 nucleotides HP15_257 and HP15_258 are separated by 83 nucleotides
HP15_256: HP15_256 - serine protease, at 245,359 to 247,575
_256
HP15_257: HP15_257 - transcriptional regulator, GntR family, at 247,582 to 248,259
_257
HP15_258: HP15_258 - phospholipase D/Transphosphatidylase, at 248,343 to 249,773
_258
Position (kb) 247
248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 246.602 kb on - strand, within HP15_256 at 246.733 kb on - strand, within HP15_256 at 246.830 kb on + strand, within HP15_256 at 246.838 kb on - strand, within HP15_256 at 246.848 kb on - strand, within HP15_256 at 246.954 kb on + strand, within HP15_256 at 246.999 kb on + strand, within HP15_256 at 247.047 kb on - strand, within HP15_256 at 247.057 kb on - strand, within HP15_256 at 247.061 kb on - strand, within HP15_256 at 247.127 kb on - strand, within HP15_256 at 247.184 kb on + strand, within HP15_256 at 247.184 kb on + strand, within HP15_256 at 247.303 kb on + strand, within HP15_256 at 247.365 kb on + strand at 247.365 kb on + strand at 247.370 kb on + strand at 247.410 kb on - strand at 247.412 kb on + strand at 247.420 kb on - strand at 247.426 kb on + strand at 247.566 kb on + strand at 247.574 kb on - strand at 247.770 kb on - strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.787 kb on + strand, within HP15_257 at 247.795 kb on - strand, within HP15_257 at 247.795 kb on - strand, within HP15_257 at 247.829 kb on + strand, within HP15_257 at 247.842 kb on - strand, within HP15_257 at 247.844 kb on + strand, within HP15_257 at 247.844 kb on + strand, within HP15_257 at 247.852 kb on - strand, within HP15_257 at 247.862 kb on - strand, within HP15_257 at 248.003 kb on + strand, within HP15_257 at 248.054 kb on + strand, within HP15_257 at 248.131 kb on - strand, within HP15_257 at 248.164 kb on - strand, within HP15_257 at 248.232 kb on + strand at 248.301 kb on - strand at 248.341 kb on + strand at 248.341 kb on + strand at 248.396 kb on - strand at 248.434 kb on + strand at 248.491 kb on - strand, within HP15_258 at 248.501 kb on - strand, within HP15_258 at 248.501 kb on - strand, within HP15_258 at 248.548 kb on + strand, within HP15_258 at 248.603 kb on - strand, within HP15_258 at 248.650 kb on + strand, within HP15_258 at 248.650 kb on + strand, within HP15_258 at 248.658 kb on - strand, within HP15_258 at 248.658 kb on - strand, within HP15_258 at 248.658 kb on - strand, within HP15_258 at 248.738 kb on + strand, within HP15_258 at 248.763 kb on + strand, within HP15_258 at 248.781 kb on - strand, within HP15_258 at 248.796 kb on - strand, within HP15_258 at 248.863 kb on + strand, within HP15_258 at 248.957 kb on - strand, within HP15_258 at 248.990 kb on - strand, within HP15_258 at 249.018 kb on + strand, within HP15_258 at 249.033 kb on + strand, within HP15_258 at 249.033 kb on + strand, within HP15_258 at 249.045 kb on + strand, within HP15_258 at 249.065 kb on + strand, within HP15_258
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 246,602 - HP15_256 0.56 -1.3 246,733 - HP15_256 0.62 +3.3 246,830 + HP15_256 0.66 +0.5 246,838 - HP15_256 0.67 +1.5 246,848 - HP15_256 0.67 +0.2 246,954 + HP15_256 0.72 -0.3 246,999 + HP15_256 0.74 -0.3 247,047 - HP15_256 0.76 +0.2 247,057 - HP15_256 0.77 +1.9 247,061 - HP15_256 0.77 -1.6 247,127 - HP15_256 0.80 +1.8 247,184 + HP15_256 0.82 -0.0 247,184 + HP15_256 0.82 +0.1 247,303 + HP15_256 0.88 -0.5 247,365 + -0.1 247,365 + +0.6 247,370 + +1.1 247,410 - +2.1 247,412 + +0.3 247,420 - +1.0 247,426 + -0.3 247,566 + -0.2 247,574 - +0.0 247,770 - HP15_257 0.28 -0.3 247,787 + HP15_257 0.30 +1.1 247,787 + HP15_257 0.30 +1.9 247,787 + HP15_257 0.30 +0.3 247,795 - HP15_257 0.31 +2.5 247,795 - HP15_257 0.31 -1.1 247,829 + HP15_257 0.36 +2.1 247,842 - HP15_257 0.38 +1.7 247,844 + HP15_257 0.39 +2.0 247,844 + HP15_257 0.39 +1.5 247,852 - HP15_257 0.40 -0.7 247,862 - HP15_257 0.41 +1.7 248,003 + HP15_257 0.62 -0.4 248,054 + HP15_257 0.70 +0.9 248,131 - HP15_257 0.81 -0.4 248,164 - HP15_257 0.86 +0.5 248,232 + +0.5 248,301 - +0.3 248,341 + -0.3 248,341 + +0.8 248,396 - +0.7 248,434 + +1.9 248,491 - HP15_258 0.10 -1.2 248,501 - HP15_258 0.11 +3.3 248,501 - HP15_258 0.11 -0.4 248,548 + HP15_258 0.14 -0.4 248,603 - HP15_258 0.18 -0.0 248,650 + HP15_258 0.21 -0.3 248,650 + HP15_258 0.21 -0.8 248,658 - HP15_258 0.22 +1.0 248,658 - HP15_258 0.22 -2.1 248,658 - HP15_258 0.22 +0.5 248,738 + HP15_258 0.28 -0.2 248,763 + HP15_258 0.29 -3.1 248,781 - HP15_258 0.31 -0.5 248,796 - HP15_258 0.32 -0.7 248,863 + HP15_258 0.36 +1.6 248,957 - HP15_258 0.43 -0.1 248,990 - HP15_258 0.45 +3.1 249,018 + HP15_258 0.47 -0.1 249,033 + HP15_258 0.48 +0.3 249,033 + HP15_258 0.48 +0.3 249,045 + HP15_258 0.49 -0.2 249,065 + HP15_258 0.50 +2.1
Or see this region's nucleotide sequence