Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_216 and HP15_217 are separated by 66 nucleotides HP15_217 and HP15_218 are separated by 122 nucleotides HP15_218 and HP15_219 are separated by 20 nucleotides HP15_219 and HP15_220 are separated by 150 nucleotides
HP15_216: HP15_216 - DNA damage response protein (yhgF like) <small>(from data)</small>, at 203,304 to 205,643
_216
HP15_217: HP15_217 - glutamate--cysteine ligase, at 205,710 to 207,263
_217
HP15_218: HP15_218 - conserved hypothetical protein, at 207,386 to 207,601
_218
HP15_219: HP15_219 - flagellar basal body-associated protein FliL, at 207,622 to 208,071
_219
HP15_220: HP15_220 - disulfide bond formation protein B, at 208,222 to 208,629
_220
Position (kb) 205
206
207
208 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 204.749 kb on + strand, within HP15_216 at 204.792 kb on + strand, within HP15_216 at 204.795 kb on - strand, within HP15_216 at 204.827 kb on + strand, within HP15_216 at 204.911 kb on + strand, within HP15_216 at 205.107 kb on - strand, within HP15_216 at 205.205 kb on - strand, within HP15_216 at 205.234 kb on - strand, within HP15_216 at 205.236 kb on - strand, within HP15_216 at 205.352 kb on + strand, within HP15_216 at 205.360 kb on - strand, within HP15_216 at 205.360 kb on - strand, within HP15_216 at 205.361 kb on - strand, within HP15_216 at 205.375 kb on + strand, within HP15_216 at 205.409 kb on + strand, within HP15_216 at 205.417 kb on - strand at 205.615 kb on - strand at 205.639 kb on + strand at 205.656 kb on + strand at 205.721 kb on - strand at 207.277 kb on - strand at 207.427 kb on + strand, within HP15_218 at 207.455 kb on - strand, within HP15_218 at 207.560 kb on - strand, within HP15_218 at 208.110 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 204,749 + HP15_216 0.62 -0.7 204,792 + HP15_216 0.64 -0.4 204,795 - HP15_216 0.64 -0.8 204,827 + HP15_216 0.65 -0.3 204,911 + HP15_216 0.69 +1.5 205,107 - HP15_216 0.77 -0.0 205,205 - HP15_216 0.81 +1.4 205,234 - HP15_216 0.82 -1.2 205,236 - HP15_216 0.83 -0.7 205,352 + HP15_216 0.88 +2.8 205,360 - HP15_216 0.88 -1.3 205,360 - HP15_216 0.88 +1.6 205,361 - HP15_216 0.88 -0.6 205,375 + HP15_216 0.89 -1.0 205,409 + HP15_216 0.90 +2.1 205,417 - +0.5 205,615 - -0.5 205,639 + -2.9 205,656 + -1.8 205,721 - +1.3 207,277 - +2.9 207,427 + HP15_218 0.19 -1.1 207,455 - HP15_218 0.32 +2.6 207,560 - HP15_218 0.81 +2.4 208,110 + +0.6
Or see this region's nucleotide sequence