Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_197 and HP15_198 are separated by 75 nucleotides HP15_198 and HP15_199 overlap by 11 nucleotides HP15_199 and HP15_200 are separated by 123 nucleotides
HP15_197: HP15_197 - YaeQ family protein, at 185,061 to 185,606
_197
HP15_198: HP15_198 - LrgA protein, at 185,682 to 186,041
_198
HP15_199: HP15_199 - LrgB family protein, at 186,031 to 186,753
_199
HP15_200: HP15_200 - peptide ABC transporter, periplasmic peptide-binding protein, at 186,877 to 188,472
_200
Position (kb) 186
187 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.059 kb on + strand at 185.064 kb on + strand at 185.072 kb on - strand at 185.072 kb on - strand at 185.089 kb on - strand at 185.155 kb on - strand, within HP15_197 at 185.185 kb on + strand, within HP15_197 at 185.186 kb on + strand, within HP15_197 at 185.187 kb on - strand, within HP15_197 at 185.265 kb on + strand, within HP15_197 at 185.290 kb on + strand, within HP15_197 at 185.322 kb on - strand, within HP15_197 at 185.324 kb on - strand, within HP15_197 at 185.351 kb on + strand, within HP15_197 at 185.351 kb on + strand, within HP15_197 at 185.421 kb on + strand, within HP15_197 at 185.462 kb on + strand, within HP15_197 at 185.574 kb on - strand at 185.586 kb on - strand at 185.628 kb on + strand at 185.628 kb on + strand at 185.628 kb on + strand at 185.628 kb on + strand at 185.628 kb on + strand at 185.636 kb on + strand at 185.636 kb on + strand at 185.636 kb on - strand at 185.636 kb on - strand at 185.640 kb on + strand at 185.644 kb on - strand at 185.654 kb on - strand at 185.672 kb on + strand at 185.680 kb on - strand at 185.805 kb on - strand, within HP15_198 at 185.847 kb on + strand, within HP15_198 at 185.848 kb on + strand, within HP15_198 at 185.979 kb on + strand, within HP15_198 at 186.054 kb on + strand at 186.054 kb on + strand at 186.085 kb on + strand at 186.112 kb on - strand, within HP15_199 at 186.157 kb on - strand, within HP15_199 at 186.161 kb on + strand, within HP15_199 at 186.420 kb on - strand, within HP15_199 at 186.429 kb on + strand, within HP15_199 at 186.430 kb on - strand, within HP15_199 at 186.517 kb on - strand, within HP15_199 at 186.565 kb on - strand, within HP15_199 at 186.584 kb on - strand, within HP15_199 at 186.627 kb on - strand, within HP15_199 at 186.721 kb on + strand at 186.812 kb on + strand at 186.812 kb on + strand at 187.018 kb on - strand at 187.055 kb on - strand, within HP15_200 at 187.079 kb on + strand, within HP15_200 at 187.079 kb on + strand, within HP15_200 at 187.185 kb on + strand, within HP15_200 at 187.237 kb on + strand, within HP15_200 at 187.247 kb on + strand, within HP15_200 at 187.319 kb on + strand, within HP15_200 at 187.532 kb on - strand, within HP15_200 at 187.555 kb on + strand, within HP15_200
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 185,059 + -1.3 185,064 + -0.6 185,072 - -0.2 185,072 - +1.3 185,089 - -0.4 185,155 - HP15_197 0.17 -1.0 185,185 + HP15_197 0.23 +0.3 185,186 + HP15_197 0.23 +0.3 185,187 - HP15_197 0.23 +1.0 185,265 + HP15_197 0.37 +0.5 185,290 + HP15_197 0.42 -0.2 185,322 - HP15_197 0.48 -1.0 185,324 - HP15_197 0.48 +0.8 185,351 + HP15_197 0.53 -0.0 185,351 + HP15_197 0.53 -1.0 185,421 + HP15_197 0.66 +1.4 185,462 + HP15_197 0.73 +1.6 185,574 - +2.5 185,586 - -0.6 185,628 + +2.3 185,628 + +1.7 185,628 + -0.3 185,628 + -0.9 185,628 + +1.4 185,636 + +2.3 185,636 + -0.7 185,636 - -0.1 185,636 - +1.3 185,640 + +1.7 185,644 - -0.2 185,654 - -0.5 185,672 + +0.8 185,680 - -0.5 185,805 - HP15_198 0.34 +0.1 185,847 + HP15_198 0.46 -0.9 185,848 + HP15_198 0.46 -0.7 185,979 + HP15_198 0.82 -0.6 186,054 + -1.5 186,054 + +1.1 186,085 + -1.1 186,112 - HP15_199 0.11 -0.5 186,157 - HP15_199 0.17 +1.6 186,161 + HP15_199 0.18 +2.0 186,420 - HP15_199 0.54 -0.6 186,429 + HP15_199 0.55 +0.4 186,430 - HP15_199 0.55 -0.8 186,517 - HP15_199 0.67 +1.0 186,565 - HP15_199 0.74 -0.5 186,584 - HP15_199 0.76 +2.5 186,627 - HP15_199 0.82 -2.0 186,721 + -1.4 186,812 + -0.0 186,812 + +1.9 187,018 - -1.0 187,055 - HP15_200 0.11 +1.6 187,079 + HP15_200 0.13 -0.8 187,079 + HP15_200 0.13 -0.5 187,185 + HP15_200 0.19 -0.3 187,237 + HP15_200 0.23 +0.7 187,247 + HP15_200 0.23 -0.8 187,319 + HP15_200 0.28 -1.6 187,532 - HP15_200 0.41 -0.5 187,555 + HP15_200 0.42 +0.2
Or see this region's nucleotide sequence