Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_10 and HP15_11 are separated by 62 nucleotides HP15_11 and HP15_12 are separated by 1 nucleotides HP15_12 and HP15_13 are separated by 143 nucleotides
HP15_10: HP15_10 - transporter, at 11,376 to 13,913
_10
HP15_11: HP15_11 - conserved hypothetical protein, at 13,976 to 14,941
_11
HP15_12: HP15_12 - enoyl-CoA hydratase/isomerase, at 14,943 to 15,755
_12
HP15_13: HP15_13 - short-chain dehydrogenase/reductase SDR, at 15,899 to 16,627
_13
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 12.992 kb on + strand, within HP15_10 at 13.008 kb on - strand, within HP15_10 at 13.008 kb on - strand, within HP15_10 at 13.011 kb on + strand, within HP15_10 at 13.011 kb on + strand, within HP15_10 at 13.016 kb on + strand, within HP15_10 at 13.019 kb on - strand, within HP15_10 at 13.024 kb on - strand, within HP15_10 at 13.093 kb on + strand, within HP15_10 at 13.106 kb on - strand, within HP15_10 at 13.107 kb on + strand, within HP15_10 at 13.115 kb on - strand, within HP15_10 at 13.115 kb on - strand, within HP15_10 at 13.188 kb on + strand, within HP15_10 at 13.268 kb on - strand, within HP15_10 at 13.312 kb on - strand, within HP15_10 at 13.331 kb on + strand, within HP15_10 at 13.337 kb on - strand, within HP15_10 at 13.339 kb on - strand, within HP15_10 at 13.339 kb on - strand, within HP15_10 at 13.339 kb on - strand, within HP15_10 at 13.344 kb on + strand, within HP15_10 at 13.352 kb on - strand, within HP15_10 at 13.365 kb on + strand, within HP15_10 at 13.365 kb on + strand, within HP15_10 at 13.398 kb on + strand, within HP15_10 at 13.455 kb on + strand, within HP15_10 at 13.462 kb on + strand, within HP15_10 at 13.462 kb on + strand, within HP15_10 at 13.463 kb on - strand, within HP15_10 at 13.467 kb on + strand, within HP15_10 at 13.467 kb on + strand, within HP15_10 at 13.467 kb on + strand, within HP15_10 at 13.475 kb on - strand, within HP15_10 at 13.475 kb on - strand, within HP15_10 at 13.512 kb on - strand, within HP15_10 at 13.589 kb on - strand, within HP15_10 at 13.599 kb on + strand, within HP15_10 at 13.599 kb on + strand, within HP15_10 at 13.599 kb on + strand, within HP15_10 at 13.675 kb on - strand at 13.766 kb on + strand at 13.883 kb on - strand at 13.896 kb on - strand at 13.933 kb on + strand at 13.941 kb on - strand at 13.941 kb on - strand at 13.976 kb on - strand at 14.148 kb on - strand, within HP15_11 at 14.245 kb on + strand, within HP15_11 at 14.271 kb on - strand, within HP15_11 at 14.443 kb on - strand, within HP15_11 at 14.487 kb on + strand, within HP15_11 at 14.535 kb on - strand, within HP15_11 at 14.554 kb on + strand, within HP15_11 at 14.562 kb on - strand, within HP15_11 at 14.566 kb on - strand, within HP15_11 at 14.578 kb on - strand, within HP15_11 at 14.583 kb on - strand, within HP15_11 at 14.583 kb on - strand, within HP15_11 at 14.620 kb on + strand, within HP15_11 at 14.707 kb on + strand, within HP15_11 at 14.715 kb on - strand, within HP15_11 at 14.754 kb on + strand, within HP15_11 at 14.796 kb on + strand, within HP15_11 at 14.847 kb on + strand at 14.872 kb on + strand at 14.873 kb on + strand at 14.879 kb on + strand at 14.880 kb on - strand at 14.903 kb on - strand at 14.918 kb on + strand at 14.920 kb on + strand at 14.935 kb on + strand at 14.940 kb on + strand at 15.103 kb on - strand, within HP15_12 at 15.157 kb on - strand, within HP15_12 at 15.235 kb on + strand, within HP15_12 at 15.235 kb on + strand, within HP15_12 at 15.312 kb on - strand, within HP15_12 at 15.315 kb on + strand, within HP15_12 at 15.315 kb on + strand, within HP15_12 at 15.354 kb on + strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.463 kb on - strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.485 kb on + strand, within HP15_12 at 15.493 kb on + strand, within HP15_12 at 15.493 kb on + strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.493 kb on - strand, within HP15_12 at 15.501 kb on - strand, within HP15_12 at 15.546 kb on + strand, within HP15_12 at 15.839 kb on + strand at 15.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 12,992 + HP15_10 0.64 +2.3 13,008 - HP15_10 0.64 +2.8 13,008 - HP15_10 0.64 -0.4 13,011 + HP15_10 0.64 -0.8 13,011 + HP15_10 0.64 +2.6 13,016 + HP15_10 0.65 +2.0 13,019 - HP15_10 0.65 -1.3 13,024 - HP15_10 0.65 -0.4 13,093 + HP15_10 0.68 -2.5 13,106 - HP15_10 0.68 +2.1 13,107 + HP15_10 0.68 +0.3 13,115 - HP15_10 0.69 -0.2 13,115 - HP15_10 0.69 -1.4 13,188 + HP15_10 0.71 -1.4 13,268 - HP15_10 0.75 -0.6 13,312 - HP15_10 0.76 -0.3 13,331 + HP15_10 0.77 -0.5 13,337 - HP15_10 0.77 +1.8 13,339 - HP15_10 0.77 +1.6 13,339 - HP15_10 0.77 -1.3 13,339 - HP15_10 0.77 +0.7 13,344 + HP15_10 0.78 -0.1 13,352 - HP15_10 0.78 -0.8 13,365 + HP15_10 0.78 -0.2 13,365 + HP15_10 0.78 -0.1 13,398 + HP15_10 0.80 +1.7 13,455 + HP15_10 0.82 +3.1 13,462 + HP15_10 0.82 +0.6 13,462 + HP15_10 0.82 -1.0 13,463 - HP15_10 0.82 -1.5 13,467 + HP15_10 0.82 -0.8 13,467 + HP15_10 0.82 +0.9 13,467 + HP15_10 0.82 -0.9 13,475 - HP15_10 0.83 -1.3 13,475 - HP15_10 0.83 -1.0 13,512 - HP15_10 0.84 -0.7 13,589 - HP15_10 0.87 -0.3 13,599 + HP15_10 0.88 -0.7 13,599 + HP15_10 0.88 +1.6 13,599 + HP15_10 0.88 -0.4 13,675 - -0.3 13,766 + +1.9 13,883 - -0.3 13,896 - -0.5 13,933 + +0.2 13,941 - -0.8 13,941 - +1.0 13,976 - -0.5 14,148 - HP15_11 0.18 +2.2 14,245 + HP15_11 0.28 +1.9 14,271 - HP15_11 0.31 +1.6 14,443 - HP15_11 0.48 +0.7 14,487 + HP15_11 0.53 -0.7 14,535 - HP15_11 0.58 -1.5 14,554 + HP15_11 0.60 -0.7 14,562 - HP15_11 0.61 +1.6 14,566 - HP15_11 0.61 +2.1 14,578 - HP15_11 0.62 +1.6 14,583 - HP15_11 0.63 -0.1 14,583 - HP15_11 0.63 +2.8 14,620 + HP15_11 0.67 +1.3 14,707 + HP15_11 0.76 +0.0 14,715 - HP15_11 0.77 -1.6 14,754 + HP15_11 0.81 -0.3 14,796 + HP15_11 0.85 -1.2 14,847 + +0.6 14,872 + -0.2 14,873 + +0.5 14,879 + +1.7 14,880 - +1.7 14,903 - +2.1 14,918 + -0.2 14,920 + -0.7 14,935 + -1.5 14,940 + +0.1 15,103 - HP15_12 0.20 -0.2 15,157 - HP15_12 0.26 -1.2 15,235 + HP15_12 0.36 +1.2 15,235 + HP15_12 0.36 -0.3 15,312 - HP15_12 0.45 -0.5 15,315 + HP15_12 0.46 -0.3 15,315 + HP15_12 0.46 +0.6 15,354 + HP15_12 0.51 -0.9 15,463 - HP15_12 0.64 +0.2 15,463 - HP15_12 0.64 -0.4 15,463 - HP15_12 0.64 +0.0 15,463 - HP15_12 0.64 +1.0 15,485 + HP15_12 0.67 +2.1 15,485 + HP15_12 0.67 -1.2 15,485 + HP15_12 0.67 +0.4 15,493 + HP15_12 0.68 +2.3 15,493 + HP15_12 0.68 -0.8 15,493 - HP15_12 0.68 +1.0 15,493 - HP15_12 0.68 +0.7 15,493 - HP15_12 0.68 +2.3 15,493 - HP15_12 0.68 +2.0 15,501 - HP15_12 0.69 +1.9 15,546 + HP15_12 0.74 -0.2 15,839 + +1.6 15,851 + -0.6
Or see this region's nucleotide sequence