Experiment: D-Alanine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_1027 and HP15_1028 are separated by 111 nucleotides HP15_1028 and HP15_1029 are separated by 4 nucleotides HP15_1029 and HP15_1030 are separated by 164 nucleotides
HP15_1027: HP15_1027 - chemotactic transducer-related protein, at 1,043,053 to 1,045,878
_1027
HP15_1028: HP15_1028 - alkylated DNA repair protein AlkB, at 1,045,990 to 1,046,640
_1028
HP15_1029: HP15_1029 - conserved hypothetical protein, membrane, at 1,046,645 to 1,047,925
_1029
HP15_1030: HP15_1030 - sulfate permease family protein, at 1,048,090 to 1,049,577
_1030
Position (kb)
1046
1047
1048 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1045.670 kb on - strand at 1045.690 kb on - strand at 1045.690 kb on - strand at 1045.692 kb on + strand at 1045.709 kb on - strand at 1045.848 kb on - strand at 1045.859 kb on + strand at 1045.892 kb on - strand at 1045.912 kb on - strand at 1045.950 kb on - strand at 1046.018 kb on + strand at 1046.128 kb on - strand, within HP15_1028 at 1046.142 kb on + strand, within HP15_1028 at 1046.150 kb on + strand, within HP15_1028 at 1046.184 kb on - strand, within HP15_1028 at 1046.184 kb on - strand, within HP15_1028 at 1046.197 kb on - strand, within HP15_1028 at 1046.202 kb on - strand, within HP15_1028 at 1046.303 kb on - strand, within HP15_1028 at 1046.394 kb on + strand, within HP15_1028 at 1046.402 kb on - strand, within HP15_1028 at 1046.419 kb on - strand, within HP15_1028 at 1046.488 kb on + strand, within HP15_1028 at 1046.581 kb on + strand at 1046.644 kb on - strand at 1046.741 kb on - strand at 1046.816 kb on + strand, within HP15_1029 at 1046.968 kb on + strand, within HP15_1029 at 1047.057 kb on + strand, within HP15_1029 at 1047.090 kb on + strand, within HP15_1029 at 1047.090 kb on + strand, within HP15_1029 at 1047.103 kb on - strand, within HP15_1029 at 1047.112 kb on - strand, within HP15_1029 at 1047.210 kb on - strand, within HP15_1029 at 1047.599 kb on + strand, within HP15_1029 at 1047.599 kb on + strand, within HP15_1029 at 1047.607 kb on - strand, within HP15_1029 at 1047.649 kb on - strand, within HP15_1029 at 1047.687 kb on + strand, within HP15_1029 at 1047.692 kb on + strand, within HP15_1029 at 1047.772 kb on - strand, within HP15_1029 at 1047.777 kb on - strand, within HP15_1029 at 1047.819 kb on - strand at 1047.822 kb on + strand at 1047.830 kb on - strand at 1047.830 kb on - strand at 1047.875 kb on + strand at 1047.920 kb on + strand at 1047.949 kb on - strand at 1048.148 kb on - strand at 1048.509 kb on - strand, within HP15_1030 at 1048.593 kb on + strand, within HP15_1030 at 1048.606 kb on + strand, within HP15_1030 at 1048.644 kb on + strand, within HP15_1030 at 1048.734 kb on + strand, within HP15_1030 at 1048.766 kb on - strand, within HP15_1030 at 1048.784 kb on - strand, within HP15_1030 at 1048.846 kb on - strand, within HP15_1030 at 1048.848 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.867 kb on + strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.875 kb on - strand, within HP15_1030 at 1048.907 kb on - strand, within HP15_1030
Per-strain Table
Position Strand Gene LocusTag Fraction D-Alanine as (N) remove 1,045,670 - +1.6 1,045,690 - +2.5 1,045,690 - +1.8 1,045,692 + +1.6 1,045,709 - +1.7 1,045,848 - -1.0 1,045,859 + -2.0 1,045,892 - +0.5 1,045,912 - -1.2 1,045,950 - -0.4 1,046,018 + +1.5 1,046,128 - HP15_1028 0.21 +0.1 1,046,142 + HP15_1028 0.23 +0.3 1,046,150 + HP15_1028 0.25 -0.2 1,046,184 - HP15_1028 0.30 +1.7 1,046,184 - HP15_1028 0.30 -2.9 1,046,197 - HP15_1028 0.32 -1.5 1,046,202 - HP15_1028 0.33 -1.4 1,046,303 - HP15_1028 0.48 -1.0 1,046,394 + HP15_1028 0.62 -1.0 1,046,402 - HP15_1028 0.63 -1.7 1,046,419 - HP15_1028 0.66 -1.2 1,046,488 + HP15_1028 0.76 -0.4 1,046,581 + +1.2 1,046,644 - -0.6 1,046,741 - +0.2 1,046,816 + HP15_1029 0.13 +0.6 1,046,968 + HP15_1029 0.25 -1.1 1,047,057 + HP15_1029 0.32 -1.2 1,047,090 + HP15_1029 0.35 +0.2 1,047,090 + HP15_1029 0.35 -0.5 1,047,103 - HP15_1029 0.36 +0.8 1,047,112 - HP15_1029 0.36 -0.3 1,047,210 - HP15_1029 0.44 -0.7 1,047,599 + HP15_1029 0.74 -0.8 1,047,599 + HP15_1029 0.74 +0.6 1,047,607 - HP15_1029 0.75 +1.7 1,047,649 - HP15_1029 0.78 -0.1 1,047,687 + HP15_1029 0.81 -0.1 1,047,692 + HP15_1029 0.82 +2.9 1,047,772 - HP15_1029 0.88 -1.1 1,047,777 - HP15_1029 0.88 -0.7 1,047,819 - +2.5 1,047,822 + +1.8 1,047,830 - -0.1 1,047,830 - +0.2 1,047,875 + -1.4 1,047,920 + -0.7 1,047,949 - +2.4 1,048,148 - +2.2 1,048,509 - HP15_1030 0.28 -0.2 1,048,593 + HP15_1030 0.34 +1.8 1,048,606 + HP15_1030 0.35 +1.7 1,048,644 + HP15_1030 0.37 +1.8 1,048,734 + HP15_1030 0.43 -0.0 1,048,766 - HP15_1030 0.45 -0.1 1,048,784 - HP15_1030 0.47 +0.5 1,048,846 - HP15_1030 0.51 +0.6 1,048,848 + HP15_1030 0.51 -0.8 1,048,867 + HP15_1030 0.52 -1.2 1,048,867 + HP15_1030 0.52 -0.9 1,048,867 + HP15_1030 0.52 -1.3 1,048,875 - HP15_1030 0.53 +1.1 1,048,875 - HP15_1030 0.53 +2.3 1,048,875 - HP15_1030 0.53 -0.8 1,048,907 - HP15_1030 0.55 -0.7
Or see this region's nucleotide sequence