Strain Fitness in Desulfovibrio vulgaris Miyazaki F around DvMF_0154

Experiment: methionine 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntDvMF_0153 and ilvH are separated by 12 nucleotidesilvH and DvMF_0155 are separated by 335 nucleotides DvMF_0153: DvMF_0153 - acetolactate synthase, large subunit, biosynthetic type (RefSeq), at 167,254 to 168,942 _0153 DvMF_0154: ilvH - acetolactate synthase 3 regulatory subunit (RefSeq), at 168,955 to 169,446 ilvH DvMF_0155: DvMF_0155 - ketol-acid reductoisomerase (RefSeq), at 169,782 to 170,774 _0155 Position (kb) 168 169 170Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 168.461 kb on - strand, within DvMF_0153at 168.461 kb on - strand, within DvMF_0153at 168.549 kb on - strand, within DvMF_0153at 168.944 kb on - strandat 169.002 kb on + strandat 169.344 kb on + strand, within ilvHat 169.429 kb on - strandat 169.802 kb on + strandat 169.994 kb on - strand, within DvMF_0155at 170.279 kb on - strand, within DvMF_0155

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Per-strain Table

Position Strand Gene LocusTag Fraction methionine 1 mM
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168,461 - DvMF_0153 0.71 -4.4
168,461 - DvMF_0153 0.71 -3.0
168,549 - DvMF_0153 0.77 -3.9
168,944 - +0.2
169,002 + -0.8
169,344 + ilvH DvMF_0154 0.79 -1.3
169,429 - -1.2
169,802 + -3.0
169,994 - DvMF_0155 0.21 -3.4
170,279 - DvMF_0155 0.50 -3.4

Or see this region's nucleotide sequence