Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3626 and Shew_3627 overlap by 4 nucleotides Shew_3627 and Shew_3628 are separated by 156 nucleotides Shew_3628 and Shew_3629 overlap by 7 nucleotides Shew_3629 and Shew_3630 are separated by 31 nucleotides Shew_3630 and Shew_3631 are separated by 58 nucleotides
Shew_3626: Shew_3626 - hypothetical protein (RefSeq), at 4,326,037 to 4,326,462
_3626
Shew_3627: Shew_3627 - XRE family transcriptional regulator (RefSeq), at 4,326,459 to 4,326,656
_3627
Shew_3628: Shew_3628 - MgtC/SapB transporter (RefSeq), at 4,326,813 to 4,327,295
_3628
Shew_3629: Shew_3629 - competence protein ComF (RefSeq), at 4,327,289 to 4,328,047
_3629
Shew_3630: Shew_3630 - bioH protein (RefSeq), at 4,328,079 to 4,328,858
_3630
Shew_3631: Shew_3631 - hypothetical protein (RefSeq), at 4,328,917 to 4,329,411
_3631
Position (kb)
4327
4328
4329 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4326.651 kb on + strand at 4326.917 kb on - strand, within Shew_3628 at 4326.969 kb on - strand, within Shew_3628 at 4327.073 kb on + strand, within Shew_3628 at 4327.525 kb on + strand, within Shew_3629 at 4327.868 kb on + strand, within Shew_3629 at 4328.056 kb on + strand at 4328.349 kb on + strand, within Shew_3630 at 4328.357 kb on - strand, within Shew_3630 at 4328.570 kb on - strand, within Shew_3630 at 4328.834 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 4,326,651 + -0.1 4,326,917 - Shew_3628 0.22 +0.6 4,326,969 - Shew_3628 0.32 +0.4 4,327,073 + Shew_3628 0.54 +0.3 4,327,525 + Shew_3629 0.31 +0.8 4,327,868 + Shew_3629 0.76 +0.1 4,328,056 + -0.1 4,328,349 + Shew_3630 0.35 -3.2 4,328,357 - Shew_3630 0.36 -3.2 4,328,570 - Shew_3630 0.63 -0.8 4,328,834 - -4.8
Or see this region's nucleotide sequence