Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3613 and Shew_3614 overlap by 20 nucleotides Shew_3614 and Shew_3615 overlap by 8 nucleotides Shew_3615 and Shew_3616 are separated by 20 nucleotides Shew_3616 and Shew_3617 are separated by 71 nucleotides
Shew_3613: Shew_3613 - general secretion pathway protein J (RefSeq), at 4,310,779 to 4,311,558
_3613
Shew_3614: Shew_3614 - general secretion pathway protein I (RefSeq), at 4,311,539 to 4,311,943
_3614
Shew_3615: Shew_3615 - general secretion pathway protein H (RefSeq), at 4,311,936 to 4,312,514
_3615
Shew_3616: Shew_3616 - general secretion pathway protein G (RefSeq), at 4,312,535 to 4,312,969
_3616
Shew_3617: Shew_3617 - general secretion pathway protein F (RefSeq), at 4,313,041 to 4,314,261
_3617
Position (kb)
4312
4313 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4311.574 kb on - strand at 4311.824 kb on - strand, within Shew_3614 at 4311.930 kb on - strand at 4311.973 kb on - strand at 4311.975 kb on + strand at 4311.975 kb on + strand at 4311.983 kb on - strand at 4311.983 kb on - strand at 4312.409 kb on - strand, within Shew_3615 at 4312.526 kb on + strand at 4312.534 kb on - strand at 4312.625 kb on - strand, within Shew_3616 at 4312.975 kb on - strand at 4313.153 kb on - strand at 4313.153 kb on - strand at 4313.301 kb on + strand, within Shew_3617 at 4313.594 kb on - strand, within Shew_3617 at 4313.817 kb on - strand, within Shew_3617
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 4,311,574 - -1.4 4,311,824 - Shew_3614 0.70 -2.7 4,311,930 - -0.5 4,311,973 - -1.5 4,311,975 + -2.4 4,311,975 + -1.7 4,311,983 - -0.4 4,311,983 - -1.3 4,312,409 - Shew_3615 0.82 +0.1 4,312,526 + -2.1 4,312,534 - -1.9 4,312,625 - Shew_3616 0.21 -1.2 4,312,975 - -2.7 4,313,153 - -2.6 4,313,153 - -0.1 4,313,301 + Shew_3617 0.21 -1.5 4,313,594 - Shew_3617 0.45 -3.9 4,313,817 - Shew_3617 0.64 -0.1
Or see this region's nucleotide sequence