Experiment: Cobalt chloride 0.04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1042 and Shew_1043 overlap by 4 nucleotides Shew_1043 and nhaR are separated by 20 nucleotides nhaR and Shew_1045 are separated by 64 nucleotides Shew_1045 and Shew_1046 overlap by 20 nucleotides Shew_1046 and Shew_1047 are separated by 8 nucleotides
Shew_1042: Shew_1042 - Na+/H+ antiporter NhaA (RefSeq), at 1,206,202 to 1,207,377
_1042
Shew_1043: Shew_1043 - hypothetical protein (RefSeq), at 1,207,374 to 1,207,757
_1043
Shew_1044: nhaR - transcriptional activator NhaR (RefSeq), at 1,207,778 to 1,208,686
nhaR
Shew_1045: Shew_1045 - hypothetical protein (RefSeq), at 1,208,751 to 1,209,002
_1045
Shew_1046: Shew_1046 - hypothetical protein (RefSeq), at 1,208,983 to 1,209,438
_1046
Shew_1047: Shew_1047 - L-aspartate oxidase (RefSeq), at 1,209,447 to 1,211,060
_1047
Position (kb)
1207
1208
1209 Strain fitness (log2 ratio)
-2
-1
0
1 at 1206.861 kb on - strand, within Shew_1042 at 1206.871 kb on - strand, within Shew_1042 at 1206.880 kb on + strand, within Shew_1042 at 1206.880 kb on + strand, within Shew_1042 at 1207.109 kb on + strand, within Shew_1042 at 1207.147 kb on - strand, within Shew_1042 at 1207.147 kb on - strand, within Shew_1042 at 1207.173 kb on - strand, within Shew_1042 at 1207.261 kb on + strand at 1207.747 kb on + strand at 1207.760 kb on + strand at 1207.865 kb on - strand at 1207.930 kb on - strand, within nhaR at 1208.162 kb on - strand, within nhaR at 1208.331 kb on + strand, within nhaR at 1208.830 kb on + strand, within Shew_1045 at 1209.010 kb on - strand at 1209.020 kb on + strand at 1209.121 kb on - strand, within Shew_1046 at 1209.177 kb on + strand, within Shew_1046 at 1209.191 kb on + strand, within Shew_1046
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.04 mM remove 1,206,861 - Shew_1042 0.56 +0.1 1,206,871 - Shew_1042 0.57 -0.4 1,206,880 + Shew_1042 0.58 +0.2 1,206,880 + Shew_1042 0.58 -1.0 1,207,109 + Shew_1042 0.77 +0.8 1,207,147 - Shew_1042 0.80 +0.1 1,207,147 - Shew_1042 0.80 +0.3 1,207,173 - Shew_1042 0.83 +0.2 1,207,261 + -0.4 1,207,747 + -0.3 1,207,760 + -0.5 1,207,865 - -0.7 1,207,930 - nhaR Shew_1044 0.17 -0.3 1,208,162 - nhaR Shew_1044 0.42 -0.8 1,208,331 + nhaR Shew_1044 0.61 -1.2 1,208,830 + Shew_1045 0.31 -2.0 1,209,010 - +0.8 1,209,020 + +0.3 1,209,121 - Shew_1046 0.30 +0.0 1,209,177 + Shew_1046 0.43 +0.2 1,209,191 + Shew_1046 0.46 -0.2
Or see this region's nucleotide sequence