Experiment: m.b. benzoic 0.32 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2583 and Dshi_2584 are separated by 324 nucleotides Dshi_2584 and Dshi_2585 are separated by 43 nucleotides Dshi_2585 and Dshi_2586 are separated by 18 nucleotides
Dshi_2583: Dshi_2583 - ribosomal protein L28 (RefSeq), at 2,749,939 to 2,750,226
_2583
Dshi_2584: Dshi_2584 - hypothetical protein (RefSeq), at 2,750,551 to 2,750,907
_2584
Dshi_2585: Dshi_2585 - protein of unknown function DUF461 (RefSeq), at 2,750,951 to 2,751,463
_2585
Dshi_2586: Dshi_2586 - HpcH/HpaI aldolase (RefSeq), at 2,751,482 to 2,752,237
_2586
Position (kb)
2750
2751
2752 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2750.616 kb on + strand, within Dshi_2584 at 2750.858 kb on - strand, within Dshi_2584 at 2750.930 kb on + strand at 2750.935 kb on + strand at 2751.220 kb on - strand, within Dshi_2585 at 2751.254 kb on + strand, within Dshi_2585 at 2751.344 kb on - strand, within Dshi_2585 at 2751.379 kb on + strand, within Dshi_2585 at 2751.421 kb on - strand at 2751.629 kb on + strand, within Dshi_2586 at 2751.648 kb on + strand, within Dshi_2586 at 2751.833 kb on + strand, within Dshi_2586 at 2752.255 kb on - strand at 2752.350 kb on - strand at 2752.444 kb on + strand at 2752.444 kb on + strand at 2752.452 kb on - strand at 2752.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. benzoic 0.32 mg/ml remove 2,750,616 + Dshi_2584 0.18 -0.6 2,750,858 - Dshi_2584 0.86 -2.6 2,750,930 + -0.2 2,750,935 + +2.4 2,751,220 - Dshi_2585 0.52 -1.0 2,751,254 + Dshi_2585 0.59 -2.3 2,751,344 - Dshi_2585 0.77 +0.4 2,751,379 + Dshi_2585 0.83 -1.6 2,751,421 - +1.4 2,751,629 + Dshi_2586 0.19 -0.3 2,751,648 + Dshi_2586 0.22 +1.0 2,751,833 + Dshi_2586 0.46 -1.9 2,752,255 - -2.9 2,752,350 - +1.4 2,752,444 + -1.2 2,752,444 + +2.0 2,752,452 - +0.4 2,752,452 - +0.4
Or see this region's nucleotide sequence