Experiment: m.b. benzoic 0.32 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1867 and Dshi_1868 are separated by 61 nucleotides Dshi_1868 and Dshi_1869 are separated by 20 nucleotides Dshi_1869 and Dshi_1870 overlap by 1 nucleotides
Dshi_1867: Dshi_1867 - glutamine synthetase catalytic region (RefSeq), at 1,946,164 to 1,947,480
_1867
Dshi_1868: Dshi_1868 - ferredoxin-dependent glutamate synthase (RefSeq), at 1,947,542 to 1,948,876
_1868
Dshi_1869: Dshi_1869 - glutamate synthase alpha subunit domain protein (RefSeq), at 1,948,897 to 1,949,574
_1869
Dshi_1870: Dshi_1870 - glutamine amidotransferase class-II (RefSeq), at 1,949,574 to 1,950,500
_1870
Position (kb)
1947
1948
1949 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1946.647 kb on + strand, within Dshi_1867 at 1946.727 kb on - strand, within Dshi_1867 at 1946.771 kb on - strand, within Dshi_1867 at 1946.793 kb on + strand, within Dshi_1867 at 1946.955 kb on + strand, within Dshi_1867 at 1946.971 kb on - strand, within Dshi_1867 at 1946.976 kb on - strand, within Dshi_1867 at 1947.006 kb on + strand, within Dshi_1867 at 1947.192 kb on - strand, within Dshi_1867 at 1947.253 kb on - strand, within Dshi_1867 at 1947.377 kb on - strand at 1947.378 kb on - strand at 1947.380 kb on + strand at 1947.468 kb on + strand at 1947.468 kb on + strand at 1947.468 kb on + strand at 1947.549 kb on - strand at 1947.568 kb on + strand at 1947.577 kb on - strand at 1947.738 kb on + strand, within Dshi_1868 at 1947.773 kb on + strand, within Dshi_1868 at 1947.944 kb on + strand, within Dshi_1868 at 1947.957 kb on - strand, within Dshi_1868 at 1948.117 kb on - strand, within Dshi_1868 at 1948.137 kb on + strand, within Dshi_1868 at 1948.289 kb on - strand, within Dshi_1868 at 1948.430 kb on + strand, within Dshi_1868 at 1948.518 kb on - strand, within Dshi_1868 at 1948.553 kb on - strand, within Dshi_1868 at 1948.719 kb on + strand, within Dshi_1868 at 1948.784 kb on - strand at 1949.064 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.139 kb on - strand, within Dshi_1869 at 1949.141 kb on + strand at 1949.364 kb on + strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.388 kb on - strand, within Dshi_1869 at 1949.481 kb on - strand, within Dshi_1869 at 1949.496 kb on + strand, within Dshi_1869 at 1949.556 kb on - strand at 1949.595 kb on + strand at 1949.603 kb on - strand at 1949.627 kb on - strand at 1949.672 kb on + strand, within Dshi_1870 at 1949.789 kb on - strand, within Dshi_1870 at 1949.830 kb on - strand, within Dshi_1870 at 1949.837 kb on - strand, within Dshi_1870
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. benzoic 0.32 mg/ml remove 1,946,647 + Dshi_1867 0.37 -1.3 1,946,727 - Dshi_1867 0.43 +0.2 1,946,771 - Dshi_1867 0.46 +0.4 1,946,793 + Dshi_1867 0.48 -0.0 1,946,955 + Dshi_1867 0.60 -3.3 1,946,971 - Dshi_1867 0.61 +1.6 1,946,976 - Dshi_1867 0.62 -1.3 1,947,006 + Dshi_1867 0.64 +1.3 1,947,192 - Dshi_1867 0.78 -1.4 1,947,253 - Dshi_1867 0.83 +2.5 1,947,377 - -0.5 1,947,378 - -0.2 1,947,380 + -3.1 1,947,468 + -0.4 1,947,468 + -0.1 1,947,468 + +1.3 1,947,549 - +3.3 1,947,568 + +2.3 1,947,577 - -1.6 1,947,738 + Dshi_1868 0.15 +1.9 1,947,773 + Dshi_1868 0.17 -0.2 1,947,944 + Dshi_1868 0.30 -2.0 1,947,957 - Dshi_1868 0.31 -0.3 1,948,117 - Dshi_1868 0.43 +2.9 1,948,137 + Dshi_1868 0.45 -0.4 1,948,289 - Dshi_1868 0.56 -2.9 1,948,430 + Dshi_1868 0.67 +1.3 1,948,518 - Dshi_1868 0.73 +0.3 1,948,553 - Dshi_1868 0.76 +1.9 1,948,719 + Dshi_1868 0.88 -0.7 1,948,784 - +2.3 1,949,064 - Dshi_1869 0.25 +0.3 1,949,139 - Dshi_1869 0.36 +0.3 1,949,139 - Dshi_1869 0.36 +2.8 1,949,141 + +0.3 1,949,364 + Dshi_1869 0.69 +0.7 1,949,388 - Dshi_1869 0.72 +1.6 1,949,388 - Dshi_1869 0.72 -0.7 1,949,481 - Dshi_1869 0.86 +0.1 1,949,496 + Dshi_1869 0.88 -2.1 1,949,556 - +0.9 1,949,595 + -0.8 1,949,603 - -1.6 1,949,627 - +0.8 1,949,672 + Dshi_1870 0.11 +0.6 1,949,789 - Dshi_1870 0.23 +1.3 1,949,830 - Dshi_1870 0.28 +0.4 1,949,837 - Dshi_1870 0.28 +0.1
Or see this region's nucleotide sequence