Experiment: m.b. benzoic 0.32 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0179 and Dshi_0180 are separated by 72 nucleotides Dshi_0180 and Dshi_0181 overlap by 4 nucleotides Dshi_0181 and Dshi_0182 are separated by 104 nucleotides Dshi_0182 and Dshi_0183 overlap by 8 nucleotides
Dshi_0179: Dshi_0179 - peptide deformylase (RefSeq), at 183,047 to 183,610
_0179
Dshi_0180: Dshi_0180 - peptide deformylase (RefSeq), at 183,683 to 184,189
_0180
Dshi_0181: Dshi_0181 - methionyl-tRNA formyltransferase (RefSeq), at 184,186 to 185,085
_0181
Dshi_0182: Dshi_0182 - ribonuclease H (RefSeq), at 185,190 to 185,663
_0182
Dshi_0183: Dshi_0183 - Methyltransferase type 11 (RefSeq), at 185,656 to 186,255
_0183
Position (kb) 184
185
186 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 183.760 kb on + strand, within Dshi_0180 at 183.827 kb on + strand, within Dshi_0180 at 183.959 kb on - strand, within Dshi_0180 at 184.057 kb on + strand, within Dshi_0180 at 184.077 kb on - strand, within Dshi_0180 at 184.090 kb on + strand, within Dshi_0180 at 184.168 kb on + strand at 184.193 kb on - strand at 185.057 kb on + strand at 185.109 kb on + strand at 185.189 kb on - strand at 185.597 kb on - strand, within Dshi_0182 at 185.678 kb on - strand at 185.801 kb on + strand, within Dshi_0183 at 185.801 kb on + strand, within Dshi_0183 at 185.844 kb on + strand, within Dshi_0183
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. benzoic 0.32 mg/ml remove 183,760 + Dshi_0180 0.15 +1.2 183,827 + Dshi_0180 0.28 -2.4 183,959 - Dshi_0180 0.54 +0.6 184,057 + Dshi_0180 0.74 -0.9 184,077 - Dshi_0180 0.78 +2.2 184,090 + Dshi_0180 0.80 -1.4 184,168 + +2.6 184,193 - -1.1 185,057 + -0.9 185,109 + +0.6 185,189 - +2.2 185,597 - Dshi_0182 0.86 +1.6 185,678 - -3.1 185,801 + Dshi_0183 0.24 -0.3 185,801 + Dshi_0183 0.24 +2.2 185,844 + Dshi_0183 0.31 -1.4
Or see this region's nucleotide sequence