Experiment: copper (II) chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1671 and Shew_1672 are separated by 35 nucleotides Shew_1672 and fabG are separated by 4 nucleotides fabG and Shew_1674 are separated by 113 nucleotides Shew_1674 and Shew_1675 are separated by 9 nucleotides
Shew_1671: Shew_1671 - enoyl-CoA hydratase/isomerase (RefSeq), at 1,920,880 to 1,922,010
_1671
Shew_1672: Shew_1672 - 3-hydroxyisobutyrate dehydrogenase (RefSeq), at 1,922,046 to 1,922,945
_1672
Shew_1673: fabG - 3-ketoacyl-(acyl-carrier-protein) reductase (RefSeq), at 1,922,950 to 1,923,708
fabG
Shew_1674: Shew_1674 - hypothetical protein (RefSeq), at 1,923,822 to 1,924,079
_1674
Shew_1675: Shew_1675 - peptidase S9 prolyl oligopeptidase (RefSeq), at 1,924,089 to 1,926,140
_1675
Position (kb)
1922
1923
1924 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1922.151 kb on + strand, within Shew_1672 at 1922.159 kb on - strand, within Shew_1672 at 1922.386 kb on - strand, within Shew_1672 at 1922.386 kb on - strand, within Shew_1672 at 1922.492 kb on + strand, within Shew_1672 at 1922.492 kb on + strand, within Shew_1672 at 1922.500 kb on - strand, within Shew_1672 at 1922.500 kb on - strand, within Shew_1672 at 1922.683 kb on - strand, within Shew_1672 at 1922.989 kb on - strand at 1923.385 kb on - strand, within fabG at 1923.400 kb on - strand, within fabG at 1924.267 kb on - strand at 1924.585 kb on - strand, within Shew_1675
Per-strain Table
Position Strand Gene LocusTag Fraction copper (II) chloride 0.5 mM remove 1,922,151 + Shew_1672 0.12 +0.3 1,922,159 - Shew_1672 0.13 -0.0 1,922,386 - Shew_1672 0.38 +0.1 1,922,386 - Shew_1672 0.38 +0.5 1,922,492 + Shew_1672 0.50 +0.8 1,922,492 + Shew_1672 0.50 -0.1 1,922,500 - Shew_1672 0.50 -0.1 1,922,500 - Shew_1672 0.50 +0.2 1,922,683 - Shew_1672 0.71 -0.4 1,922,989 - +2.0 1,923,385 - fabG Shew_1673 0.57 -0.3 1,923,400 - fabG Shew_1673 0.59 -2.2 1,924,267 - +0.3 1,924,585 - Shew_1675 0.24 +0.3
Or see this region's nucleotide sequence