Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3120 and DvMF_3121 overlap by 4 nucleotides DvMF_3121 and DvMF_3122 overlap by 1 nucleotides DvMF_3122 and DvMF_3123 are separated by 60 nucleotides
DvMF_3120: DvMF_3120 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 3,940,279 to 3,941,010
_3120
DvMF_3121: DvMF_3121 - putative lipoprotein (RefSeq), at 3,941,007 to 3,941,678
_3121
DvMF_3122: DvMF_3122 - Imidazoleglycerol-phosphate dehydratase (RefSeq), at 3,941,678 to 3,942,268
_3122
DvMF_3123: DvMF_3123 - Sec-independent protein translocase, TatC subunit (RefSeq), at 3,942,329 to 3,943,123
_3123
Position (kb)
3941
3942 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 3940.153 kb on + strand at 3940.297 kb on + strand at 3940.670 kb on + strand, within DvMF_3120 at 3941.044 kb on + strand at 3941.079 kb on - strand, within DvMF_3121 at 3941.293 kb on + strand, within DvMF_3121 at 3941.311 kb on - strand, within DvMF_3121 at 3941.609 kb on + strand, within DvMF_3121 at 3941.702 kb on + strand at 3941.748 kb on - strand, within DvMF_3122 at 3941.880 kb on + strand, within DvMF_3122 at 3942.106 kb on + strand, within DvMF_3122 at 3942.350 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 3,940,153 + -0.4 3,940,297 + -5.4 3,940,670 + DvMF_3120 0.53 -5.3 3,941,044 + -5.9 3,941,079 - DvMF_3121 0.11 -4.7 3,941,293 + DvMF_3121 0.43 -4.7 3,941,311 - DvMF_3121 0.45 -4.7 3,941,609 + DvMF_3121 0.90 -2.8 3,941,702 + -3.9 3,941,748 - DvMF_3122 0.12 -3.5 3,941,880 + DvMF_3122 0.34 -4.6 3,942,106 + DvMF_3122 0.72 -4.2 3,942,350 - -0.3
Or see this region's nucleotide sequence