Experiment: L-Proline (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4288 and Psest_4289 are separated by 58 nucleotides Psest_4289 and Psest_4290 are separated by 132 nucleotides Psest_4290 and Psest_4291 are separated by 62 nucleotides
Psest_4288: Psest_4288 - Glutamate--putrescine ligase (EC 6.3.1.11) (from data) , at 4,480,916 to 4,482,289
_4288
Psest_4289: Psest_4289 - Transcriptional regulator, at 4,482,348 to 4,482,998
_4289
Psest_4290: Psest_4290 - N-carbamoylputrescine amidase, at 4,483,131 to 4,484,012
_4290
Psest_4291: Psest_4291 - agmatine deiminase, at 4,484,075 to 4,485,181
_4291
Position (kb)
4483
4484
4485 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4482.177 kb on - strand at 4482.177 kb on - strand at 4482.191 kb on + strand at 4482.191 kb on + strand at 4482.192 kb on - strand at 4482.192 kb on - strand at 4482.192 kb on - strand at 4482.192 kb on - strand at 4482.192 kb on - strand at 4482.192 kb on - strand at 4482.263 kb on + strand at 4482.263 kb on + strand at 4482.264 kb on - strand at 4482.264 kb on - strand at 4482.264 kb on - strand at 4482.342 kb on - strand at 4482.472 kb on + strand, within Psest_4289 at 4482.472 kb on + strand, within Psest_4289 at 4482.472 kb on + strand, within Psest_4289 at 4482.473 kb on - strand, within Psest_4289 at 4482.473 kb on - strand, within Psest_4289 at 4482.473 kb on - strand, within Psest_4289 at 4482.473 kb on - strand, within Psest_4289 at 4482.473 kb on - strand, within Psest_4289 at 4482.474 kb on + strand, within Psest_4289 at 4482.474 kb on + strand, within Psest_4289 at 4482.474 kb on + strand, within Psest_4289 at 4482.475 kb on - strand, within Psest_4289 at 4482.475 kb on - strand, within Psest_4289 at 4482.516 kb on + strand, within Psest_4289 at 4482.517 kb on - strand, within Psest_4289 at 4482.517 kb on - strand, within Psest_4289 at 4482.670 kb on + strand, within Psest_4289 at 4482.672 kb on + strand, within Psest_4289 at 4482.672 kb on + strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.673 kb on - strand, within Psest_4289 at 4482.868 kb on - strand, within Psest_4289 at 4482.868 kb on - strand, within Psest_4289 at 4482.868 kb on - strand, within Psest_4289 at 4482.889 kb on - strand, within Psest_4289 at 4482.954 kb on + strand at 4482.954 kb on + strand at 4482.954 kb on + strand at 4482.954 kb on + strand at 4482.955 kb on - strand at 4482.967 kb on - strand at 4483.003 kb on + strand at 4483.007 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.047 kb on + strand at 4483.070 kb on + strand at 4483.071 kb on - strand at 4483.278 kb on + strand, within Psest_4290 at 4483.278 kb on + strand, within Psest_4290 at 4483.279 kb on - strand, within Psest_4290 at 4483.279 kb on - strand, within Psest_4290 at 4483.279 kb on - strand, within Psest_4290 at 4483.306 kb on - strand, within Psest_4290 at 4483.518 kb on + strand, within Psest_4290 at 4483.518 kb on + strand, within Psest_4290 at 4483.518 kb on + strand, within Psest_4290 at 4483.519 kb on - strand, within Psest_4290 at 4483.521 kb on + strand, within Psest_4290 at 4483.521 kb on + strand, within Psest_4290 at 4483.521 kb on + strand, within Psest_4290 at 4483.522 kb on - strand, within Psest_4290 at 4483.522 kb on - strand, within Psest_4290 at 4483.522 kb on - strand, within Psest_4290 at 4483.522 kb on - strand, within Psest_4290 at 4483.560 kb on - strand, within Psest_4290 at 4483.969 kb on - strand at 4484.069 kb on + strand at 4484.069 kb on + strand at 4484.069 kb on + strand at 4484.069 kb on + strand at 4484.069 kb on + strand at 4484.069 kb on + strand at 4484.070 kb on - strand at 4484.070 kb on - strand at 4484.070 kb on - strand at 4484.070 kb on - strand at 4484.074 kb on + strand at 4484.074 kb on + strand at 4484.074 kb on + strand at 4484.075 kb on - strand at 4484.089 kb on + strand at 4484.111 kb on + strand at 4484.279 kb on + strand, within Psest_4291 at 4484.279 kb on + strand, within Psest_4291 at 4484.279 kb on + strand, within Psest_4291 at 4484.280 kb on - strand, within Psest_4291 at 4484.280 kb on - strand, within Psest_4291 at 4484.450 kb on + strand, within Psest_4291 at 4484.504 kb on + strand, within Psest_4291 at 4484.653 kb on - strand, within Psest_4291 at 4484.875 kb on + strand, within Psest_4291 at 4484.875 kb on + strand, within Psest_4291 at 4484.875 kb on + strand, within Psest_4291 at 4484.875 kb on + strand, within Psest_4291 at 4484.875 kb on + strand, within Psest_4291 at 4484.876 kb on - strand, within Psest_4291 at 4484.876 kb on - strand, within Psest_4291 at 4484.876 kb on - strand, within Psest_4291 at 4484.984 kb on + strand, within Psest_4291 at 4484.985 kb on - strand, within Psest_4291 at 4484.985 kb on - strand, within Psest_4291
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (C) remove 4,482,177 - -1.5 4,482,177 - +0.4 4,482,191 + -1.2 4,482,191 + -0.5 4,482,192 - +0.2 4,482,192 - +0.4 4,482,192 - -0.6 4,482,192 - -0.3 4,482,192 - +0.4 4,482,192 - +0.2 4,482,263 + +0.5 4,482,263 + -0.9 4,482,264 - -0.6 4,482,264 - -1.2 4,482,264 - +1.0 4,482,342 - -2.2 4,482,472 + Psest_4289 0.19 +0.4 4,482,472 + Psest_4289 0.19 +0.6 4,482,472 + Psest_4289 0.19 -0.9 4,482,473 - Psest_4289 0.19 +0.5 4,482,473 - Psest_4289 0.19 -1.5 4,482,473 - Psest_4289 0.19 -0.1 4,482,473 - Psest_4289 0.19 +0.8 4,482,473 - Psest_4289 0.19 -0.2 4,482,474 + Psest_4289 0.19 +0.3 4,482,474 + Psest_4289 0.19 -0.2 4,482,474 + Psest_4289 0.19 +0.1 4,482,475 - Psest_4289 0.20 +0.9 4,482,475 - Psest_4289 0.20 -0.4 4,482,516 + Psest_4289 0.26 -1.6 4,482,517 - Psest_4289 0.26 -1.1 4,482,517 - Psest_4289 0.26 -0.4 4,482,670 + Psest_4289 0.49 +0.4 4,482,672 + Psest_4289 0.50 -0.3 4,482,672 + Psest_4289 0.50 -0.5 4,482,673 - Psest_4289 0.50 -0.9 4,482,673 - Psest_4289 0.50 -0.9 4,482,673 - Psest_4289 0.50 -0.5 4,482,673 - Psest_4289 0.50 -0.2 4,482,673 - Psest_4289 0.50 -1.8 4,482,673 - Psest_4289 0.50 +0.9 4,482,673 - Psest_4289 0.50 -0.1 4,482,673 - Psest_4289 0.50 +0.0 4,482,868 - Psest_4289 0.80 +0.4 4,482,868 - Psest_4289 0.80 +0.9 4,482,868 - Psest_4289 0.80 -0.5 4,482,889 - Psest_4289 0.83 -1.1 4,482,954 + -0.5 4,482,954 + -1.5 4,482,954 + -0.7 4,482,954 + -2.0 4,482,955 - +0.0 4,482,967 - -0.0 4,483,003 + +0.2 4,483,007 + -1.0 4,483,047 + -0.5 4,483,047 + +0.3 4,483,047 + +0.0 4,483,047 + -0.2 4,483,047 + -0.3 4,483,047 + -0.9 4,483,047 + -0.6 4,483,047 + -1.0 4,483,070 + +0.4 4,483,071 - -0.4 4,483,278 + Psest_4290 0.17 -0.7 4,483,278 + Psest_4290 0.17 -1.6 4,483,279 - Psest_4290 0.17 -1.7 4,483,279 - Psest_4290 0.17 -0.4 4,483,279 - Psest_4290 0.17 -1.1 4,483,306 - Psest_4290 0.20 +0.1 4,483,518 + Psest_4290 0.44 -0.6 4,483,518 + Psest_4290 0.44 +0.8 4,483,518 + Psest_4290 0.44 -0.4 4,483,519 - Psest_4290 0.44 -2.1 4,483,521 + Psest_4290 0.44 -0.3 4,483,521 + Psest_4290 0.44 -1.6 4,483,521 + Psest_4290 0.44 -0.6 4,483,522 - Psest_4290 0.44 -0.5 4,483,522 - Psest_4290 0.44 -0.4 4,483,522 - Psest_4290 0.44 -3.0 4,483,522 - Psest_4290 0.44 -1.1 4,483,560 - Psest_4290 0.49 -0.9 4,483,969 - -1.3 4,484,069 + -0.2 4,484,069 + +0.2 4,484,069 + -0.3 4,484,069 + -0.0 4,484,069 + -0.8 4,484,069 + -1.6 4,484,070 - -0.5 4,484,070 - -0.2 4,484,070 - -0.6 4,484,070 - +0.9 4,484,074 + -1.0 4,484,074 + -1.4 4,484,074 + -0.5 4,484,075 - +0.4 4,484,089 + -0.9 4,484,111 + -1.8 4,484,279 + Psest_4291 0.18 +0.3 4,484,279 + Psest_4291 0.18 -0.9 4,484,279 + Psest_4291 0.18 -0.5 4,484,280 - Psest_4291 0.19 -0.5 4,484,280 - Psest_4291 0.19 -0.2 4,484,450 + Psest_4291 0.34 -2.5 4,484,504 + Psest_4291 0.39 +0.5 4,484,653 - Psest_4291 0.52 -0.1 4,484,875 + Psest_4291 0.72 -0.9 4,484,875 + Psest_4291 0.72 -1.0 4,484,875 + Psest_4291 0.72 -0.5 4,484,875 + Psest_4291 0.72 -1.6 4,484,875 + Psest_4291 0.72 -0.4 4,484,876 - Psest_4291 0.72 +0.4 4,484,876 - Psest_4291 0.72 -2.1 4,484,876 - Psest_4291 0.72 -1.0 4,484,984 + Psest_4291 0.82 -1.2 4,484,985 - Psest_4291 0.82 -0.1 4,484,985 - Psest_4291 0.82 -1.9
Or see this region's nucleotide sequence