Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2116 and Shewana3_2117 are separated by 77 nucleotides Shewana3_2117 and Shewana3_2118 are separated by 59 nucleotides Shewana3_2118 and Shewana3_2119 overlap by 8 nucleotides
Shewana3_2116: Shewana3_2116 - universal stress protein UspE (RefSeq), at 2,539,697 to 2,540,629
_2116
Shewana3_2117: Shewana3_2117 - PP-loop domain-containing protein (RefSeq), at 2,540,707 to 2,541,675
_2117
Shewana3_2118: Shewana3_2118 - hypothetical protein (RefSeq), at 2,541,735 to 2,542,358
_2118
Shewana3_2119: Shewana3_2119 - mannosyl-glycoprotein endo-beta-N-acetylglucosamidase (RefSeq), at 2,542,351 to 2,543,163
_2119
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-1
0
1 at 2540.386 kb on - strand, within Shewana3_2116 at 2541.020 kb on + strand, within Shewana3_2117 at 2541.429 kb on + strand, within Shewana3_2117 at 2541.749 kb on + strand at 2541.823 kb on - strand, within Shewana3_2118 at 2541.856 kb on - strand, within Shewana3_2118 at 2541.876 kb on - strand, within Shewana3_2118 at 2542.008 kb on + strand, within Shewana3_2118 at 2542.053 kb on - strand, within Shewana3_2118 at 2542.568 kb on - strand, within Shewana3_2119 at 2542.601 kb on + strand, within Shewana3_2119
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,540,386 - Shewana3_2116 0.74 -1.5 2,541,020 + Shewana3_2117 0.32 +1.5 2,541,429 + Shewana3_2117 0.75 +0.9 2,541,749 + -0.6 2,541,823 - Shewana3_2118 0.14 +0.6 2,541,856 - Shewana3_2118 0.19 -0.4 2,541,876 - Shewana3_2118 0.23 +0.6 2,542,008 + Shewana3_2118 0.44 +0.5 2,542,053 - Shewana3_2118 0.51 +0.7 2,542,568 - Shewana3_2119 0.27 -1.1 2,542,601 + Shewana3_2119 0.31 -1.1
Or see this region's nucleotide sequence