Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0412 and Shewana3_0413 are separated by 147 nucleotides Shewana3_0413 and Shewana3_0414 are separated by 34 nucleotides Shewana3_0414 and Shewana3_0415 are separated by 107 nucleotides Shewana3_0415 and coaE overlap by 8 nucleotides
Shewana3_0412: Shewana3_0412 - hypothetical protein (RefSeq), at 471,568 to 472,104
_0412
Shewana3_0413: Shewana3_0413 - mutator MutT protein (RefSeq), at 472,252 to 472,650
_0413
Shewana3_0414: Shewana3_0414 - hypothetical protein (RefSeq), at 472,685 to 472,894
_0414
Shewana3_0415: Shewana3_0415 - hypothetical protein (RefSeq), at 473,002 to 473,736
_0415
Shewana3_0416: coaE - dephospho-CoA kinase (RefSeq), at 473,729 to 474,346
coaE
Position (kb) 472
473 Strain fitness (log2 ratio) -2
-1
0
1 at 471.725 kb on + strand, within Shewana3_0412 at 471.969 kb on + strand, within Shewana3_0412 at 472.172 kb on - strand at 472.445 kb on - strand, within Shewana3_0413 at 472.503 kb on - strand, within Shewana3_0413 at 472.622 kb on - strand at 472.710 kb on + strand, within Shewana3_0414 at 472.744 kb on + strand, within Shewana3_0414 at 472.984 kb on - strand at 472.991 kb on - strand at 473.042 kb on + strand at 473.072 kb on - strand at 473.219 kb on - strand, within Shewana3_0415 at 473.651 kb on + strand, within Shewana3_0415 at 473.705 kb on + strand at 473.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 471,725 + Shewana3_0412 0.29 -2.2 471,969 + Shewana3_0412 0.75 -1.2 472,172 - -0.3 472,445 - Shewana3_0413 0.48 +0.8 472,503 - Shewana3_0413 0.63 -1.7 472,622 - +0.6 472,710 + Shewana3_0414 0.12 +0.7 472,744 + Shewana3_0414 0.28 -0.7 472,984 - -1.6 472,991 - -0.7 473,042 + -0.2 473,072 - +0.7 473,219 - Shewana3_0415 0.30 +0.9 473,651 + Shewana3_0415 0.88 +0.6 473,705 + +0.7 473,715 + +0.1
Or see this region's nucleotide sequence