Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2200 and hisH overlap by 4 nucleotides hisH and Shew_2202 overlap by 4 nucleotides Shew_2202 and Shew_2203 overlap by 19 nucleotides Shew_2203 and Shew_2204 are separated by 28 nucleotides
Shew_2200: Shew_2200 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,560,961 to 2,562,031
_2200
Shew_2201: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,562,028 to 2,562,702
hisH
Shew_2202: Shew_2202 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,562,699 to 2,563,436
_2202
Shew_2203: Shew_2203 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,563,418 to 2,564,191
_2203
Shew_2204: Shew_2204 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,564,220 to 2,564,840
_2204
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-1
0
1 at 2561.750 kb on + strand, within Shew_2200 at 2561.758 kb on - strand, within Shew_2200 at 2562.347 kb on + strand, within hisH at 2562.722 kb on + strand at 2562.800 kb on + strand, within Shew_2202 at 2563.591 kb on - strand, within Shew_2203 at 2563.902 kb on + strand, within Shew_2203 at 2563.973 kb on - strand, within Shew_2203 at 2564.354 kb on - strand, within Shew_2204
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,561,750 + Shew_2200 0.74 -0.0 2,561,758 - Shew_2200 0.74 +0.3 2,562,347 + hisH Shew_2201 0.47 -0.1 2,562,722 + +0.5 2,562,800 + Shew_2202 0.14 +1.2 2,563,591 - Shew_2203 0.22 +0.3 2,563,902 + Shew_2203 0.63 +0.2 2,563,973 - Shew_2203 0.72 +1.4 2,564,354 - Shew_2204 0.22 +0.2
Or see this region's nucleotide sequence