Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1123 and trmB are separated by 235 nucleotides trmB and Shew_1125 are separated by 30 nucleotides Shew_1125 and Shew_1126 overlap by 1 nucleotides
Shew_1123: Shew_1123 - A/G-specific adenine glycosylase (RefSeq), at 1,298,053 to 1,299,159
_1123
Shew_1124: trmB - tRNA (guanine-N(7))-methyltransferase (RefSeq), at 1,299,395 to 1,300,111
trmB
Shew_1125: Shew_1125 - hypothetical protein (RefSeq), at 1,300,142 to 1,300,465
_1125
Shew_1126: Shew_1126 - glutaminase (RefSeq), at 1,300,465 to 1,301,379
_1126
Position (kb) 1299
1300
1301 Strain fitness (log2 ratio) -2
-1
0
1 at 1298.533 kb on + strand, within Shew_1123 at 1298.781 kb on - strand, within Shew_1123 at 1298.920 kb on + strand, within Shew_1123 at 1298.943 kb on + strand, within Shew_1123 at 1298.944 kb on + strand, within Shew_1123 at 1299.009 kb on + strand, within Shew_1123 at 1299.419 kb on - strand at 1299.464 kb on - strand at 1299.740 kb on - strand, within trmB at 1299.801 kb on + strand, within trmB at 1300.247 kb on - strand, within Shew_1125
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,298,533 + Shew_1123 0.43 +0.5 1,298,781 - Shew_1123 0.66 +0.3 1,298,920 + Shew_1123 0.78 -0.1 1,298,943 + Shew_1123 0.80 +0.9 1,298,944 + Shew_1123 0.80 -0.0 1,299,009 + Shew_1123 0.86 +0.4 1,299,419 - -0.1 1,299,464 - -0.0 1,299,740 - trmB Shew_1124 0.48 -1.9 1,299,801 + trmB Shew_1124 0.57 -0.5 1,300,247 - Shew_1125 0.32 -0.1
Or see this region's nucleotide sequence