Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0284 and Shew_0285 are separated by 28 nucleotides Shew_0285 and Shew_0286 are separated by 197 nucleotides
Shew_0284: Shew_0284 - hypothetical protein (RefSeq), at 302,166 to 303,491
_0284
Shew_0285: Shew_0285 - diguanylate cyclase (RefSeq), at 303,520 to 304,401
_0285
Shew_0286: Shew_0286 - extracellular solute-binding protein (RefSeq), at 304,599 to 305,408
_0286
Position (kb)
303
304
305 Strain fitness (log2 ratio)
-2
-1
0
1 at 302.621 kb on + strand, within Shew_0284 at 302.650 kb on - strand, within Shew_0284 at 302.698 kb on + strand, within Shew_0284 at 302.859 kb on - strand, within Shew_0284 at 303.036 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.046 kb on + strand, within Shew_0284 at 303.257 kb on - strand, within Shew_0284 at 303.279 kb on + strand, within Shew_0284 at 303.388 kb on + strand at 303.542 kb on - strand at 303.561 kb on + strand at 303.584 kb on - strand at 303.584 kb on + strand at 303.784 kb on - strand, within Shew_0285 at 304.174 kb on + strand, within Shew_0285 at 304.786 kb on + strand, within Shew_0286 at 304.794 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.014 kb on - strand, within Shew_0286 at 305.373 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 302,621 + Shew_0284 0.34 -0.3 302,650 - Shew_0284 0.37 +0.5 302,698 + Shew_0284 0.40 +0.7 302,859 - Shew_0284 0.52 +1.0 303,036 + Shew_0284 0.66 -0.5 303,046 + Shew_0284 0.66 +0.8 303,046 + Shew_0284 0.66 -0.1 303,046 + Shew_0284 0.66 +0.4 303,257 - Shew_0284 0.82 -0.2 303,279 + Shew_0284 0.84 -0.3 303,388 + +0.1 303,542 - +0.1 303,561 + +0.1 303,584 - +0.4 303,584 + +0.1 303,784 - Shew_0285 0.30 -1.2 304,174 + Shew_0285 0.74 -0.5 304,786 + Shew_0286 0.23 -0.1 304,794 - Shew_0286 0.24 -1.8 305,014 - Shew_0286 0.51 +0.5 305,014 - Shew_0286 0.51 -0.2 305,373 + -0.2
Or see this region's nucleotide sequence